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4 hits found for Immunity repressor

SASDMK3 – Mycobacterium phage TipsytheTRex apo protein

Immunity repressor experimental SAS data
BILBOMD model
Sample: Immunity repressor monomer, 24 kDa Mycobacterium phage TipsytheTRex protein
Buffer: 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10
...immunity repressor utilizes two domains to recognize an asymmetric DNA sequence. Nat Commun 13(1):4105 (2022)
McGinnis RJ, Brambley CA, Stamey B, Green WC, Gragg KN, Cafferty ER, Terwilliger TC, Hammel M, Hollis TJ, Miller JM, Gainey MD, Wallen JR
RgGuinier 2.3 nm
Dmax 9.0 nm
VolumePorod 43 nm3

SASDML3 – ...immunity repressor bound to 24mer DNA

Immunity repressor24mer dsDNA experimental SAS data
BILBOMD model
Sample: Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein
24mer dsDNA monomer, 15 kDa DNA
Buffer: 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10
...immunity repressor utilizes two domains to recognize an asymmetric DNA sequence. Nat Commun 13(1):4105 (2022)
McGinnis RJ, Brambley CA, Stamey B, Green WC, Gragg KN, Cafferty ER, Terwilliger TC, Hammel M, Hollis TJ, Miller JM, Gainey MD, Wallen JR
RgGuinier 2.4 nm
Dmax 9.0 nm
VolumePorod 43 nm3

SASDMM3 – ...immunity repressor mixed with 13mer DNA

Immunity repressor13mer dsDNA experimental SAS data
BILBOMD model
Sample: Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein
13mer dsDNA monomer, 8 kDa DNA
Buffer: 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 10
...immunity repressor utilizes two domains to recognize an asymmetric DNA sequence. Nat Commun 13(1):4105 (2022)
McGinnis RJ, Brambley CA, Stamey B, Green WC, Gragg KN, Cafferty ER, Terwilliger TC, Hammel M, Hollis TJ, Miller JM, Gainey MD, Wallen JR
RgGuinier 2.0 nm
Dmax 8.5 nm
VolumePorod 18 nm3

SASDLS7 – ...immunity repressor bound to DNA

Immunity repressor21mer dsDNA experimental SAS data
BILBOMD model
Sample: Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein
21mer dsDNA monomer, 13 kDa DNA
Buffer: 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10
...immunity repressor utilizes two domains to recognize an asymmetric DNA sequence. Nat Commun 13(1):4105 (2022)
McGinnis RJ, Brambley CA, Stamey B, Green WC, Gragg KN, Cafferty ER, Terwilliger TC, Hammel M, Hollis TJ, Miller JM, Gainey MD, Wallen JR
RgGuinier 2.3 nm
Dmax 7.4 nm
VolumePorod 41 nm3