The N Terminus of the Vaccinia Virus Protein F1L Is an Intrinsically Unstructured Region That Is Not Involved in Apoptosis Regulation.

Caria S, Marshall B, Burton RL, Campbell S, Pantaki-Eimany D, Hawkins CJ, Barry M, Kvansakul M, J Biol Chem 291(28):14600-8 (2016) Europe PMC

SASDBV4 – Vaccinia virus MVA F1L antiapoptotic Bcl-2 viral protein

MVA F1L antiapoptotic Bcl-2 viral protein
MWexperimental 52 kDa
MWexpected 51 kDa
VPorod 74 nm3
log I(s) 7.35×10-2 7.35×10-3 7.35×10-4 7.35×10-5
MVA F1L antiapoptotic Bcl-2 viral protein small angle scattering data  s, nm-1
ln I(s)
MVA F1L antiapoptotic Bcl-2 viral protein Guinier plot ln 7.35×10-2 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
MVA F1L antiapoptotic Bcl-2 viral protein Kratky plot 1.104 0 √3 sRg
p(r)
MVA F1L antiapoptotic Bcl-2 viral protein pair distance distribution function Rg: 3.7 nm 0 Dmax: 16.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
MVA F1L antiapoptotic Bcl-2 viral protein BUNCH model
log I(s)
 s, nm-1
MVA F1L antiapoptotic Bcl-2 viral protein CORAL model
Synchrotron SAXS data from solutions of Vaccinia virus MVA F1L antiapoptotic Bcl-2 viral protein in 25 mM HEPES, 150 mM NaCl, 5 mM DTT, pH 7.5 were collected on the SAXS/WAXS camera on the storage ring Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m (I(s) vs s = 4π sin θ/λ, where 2θ is the scattering angle). Solute concentrations ranging between 0.1 and 3.6 mg/ml were measured. 18 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected were obtained from a single concentration scattering curve.

Wavelength = UNKNOWN. Cell temperature = UNKNOWN

MVA F1L antiapoptotic Bcl-2 viral protein (MVA F1L (Protein F1))
Mol. type   Protein
Organism   Vaccinia virus
Olig. state   Dimer
Mon. MW   25.3 kDa
 
UniProt   O57173 (1-202)
Sequence   FASTA