The N-terminus of obscurin is flexible in solution.

Mauriello GE, Moncure GE, Nowzari RA, Miller CJ, Wright NT, Proteins (2022) Europe PMC

SASDNW7 – Obscurin Ig domains 12/13, short at neutral pH

Obscurin Ig domains 12/13 short
MWexperimental 21 kDa
MWexpected 21 kDa
VPorod 25 nm3
log I(s) 4.92×10-1 4.92×10-2 4.92×10-3 4.92×10-4
Obscurin Ig domains 12/13 short small angle scattering data  s, nm-1
ln I(s)
Obscurin Ig domains 12/13 short Guinier plot ln 4.93×10-1 Rg: 2.5 nm 0 (2.5 nm)-2 s2
(sRg)2I(s)/I(0)
Obscurin Ig domains 12/13 short Kratky plot 1.104 0 √3 sRg
Dmax: 9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Obscurin Ig domains 12/13 short MULTIFOXS model
Synchrotron SAXS data from solutions of Obscurin Ig domains 12/13, short at neutral pH in 20 mM Tris, 50 mM NaCl, 0.35 mM NaN3, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample concentration: UNKNOWN. Experimental temperature: UNKNOWN. X-ray exposure time: UNKNOWN.

Obscurin Ig domains 12/13 short (Obscurin Ig 12-13 Δ)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   20.7 kDa
 
UniProt   Q5VST9 (1071-1251)
Sequence   FASTA