| MWI(0) | 66 | kDa |
| MWexpected | 73 | kDa |
| VPorod | 130 | nm3 |
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log I(s)
9.84×101
9.84×100
9.84×10-1
9.84×10-2
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s, nm-1
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Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae.
Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A, MBio 5(1):e01120-13 (2014) Europe PMCSASDBG4 – Prophage LytA with choline
Prophage Lytic Amidase with choline|
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Fits and models
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Synchrotron SAXS
data from solutions of
Prophage LytA with choline
in
20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a Pilatus 1M-W detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2 and 10 mg/ml were measured
at 10°C.
Eight successive
15 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
SAXS data demonstrate that the wild type (SASDBF4) and bacteriophage LytA are similar in shape and that both form dimers in solution in the presence of choline.
Tags:
X33
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