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14 hits found for Pyrococcus furiosus sR26 substrate D

...DGV2 – ...Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D' and 2H Fibrillarin (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: ...D2O, pH: 6.6
Experiment: SANS data collected at KWS1, FRM2 on 2015 May 24
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 87 nm3

...DGW2 – ...Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D' and 2H Nop5 (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: ...D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2015 Sep 21
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 210 nm3

...DGX2 – ...Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H Nop5, 1H st-sR26 RNA, 1H substrate D' and 2H L7Ae (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: ...D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2015 Nov 23
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.0 nm
Dmax 13.3 nm
VolumePorod 42 nm3

...DGY2 – ...Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H Nop5, 1H L7Ae, 1H substrate D' and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: ...D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jul 21
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 52 nm3

...DGZ2 – ...Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H substrate D', 2H Fibrillarin and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: ...D2O, pH: 6.6
Experiment: SANS data collected at KWS1, FRM2 on 2015 May 23
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.8 nm
Dmax 16.6 nm
VolumePorod 82 nm3

...DG23 – ...Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H L7Ae, 1H substrate D', 2H(70%) Nop5 and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: ...D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Apr 8
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.0 nm
Dmax 13.8 nm
VolumePorod 214 nm3

...DG33 – ...Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H L7Ae, 1H substrate D, 2H(70%) Nop5 and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: ...D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Apr 8
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.0 nm
Dmax 13.9 nm
VolumePorod 231 nm3

...DG43 – ...Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D and 2H Fibrillarin (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: ...D2O, pH: 6.6
Experiment: SANS data collected at KWS1, FRM2 on 2015 May 24
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.9 nm
Dmax 16.6 nm
VolumePorod 65 nm3

...DG53 – ...Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D and 2H Nop5 (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: ...D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2015 Sep 21
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 222 nm3

...DG63 – ...Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H Nop5, 1H st-sR26 RNA, 1H substrate D and 2H L7Ae (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: ...D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2015 Nov 23
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 3.9 nm
Dmax 12.5 nm
VolumePorod 49 nm3

...DG73 – ...Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H Nop5, 1H L7Ae, 1H substrate D and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: ...D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jul 21
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 25 nm3

...DG83 – ...Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H substrate D, 2H Fibrillarin and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: ...D2O, pH: 6.6
Experiment: SANS data collected at KWS1, FRM2 on 2015 May 23
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 5.1 nm
Dmax 15.5 nm
VolumePorod 125 nm3

...DG93 – Pyrococcus furiosus Box C/D sRNP reconstituted with st-sR6 sRNA bound to substrate D' (SAXS data)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl, pH: 6.6
Experiment: SAXS data collected at BM29, ESRF on 2015 Jun 13
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.7 nm
Dmax 15.8 nm
VolumePorod 346 nm3

...DGA3 – Pyrococcus furiosus Box C/D sRNP reconstituted with st-sR6 sRNA bound to substrate D (SAXS data)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
...d protein dimer, 94 kDa Pyrococcus furiosus protein
pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl, pH: 6.6
Experiment: SAXS data collected at BM29, ESRF on 2015 Jul 13
...de sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
...dei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 5.0 nm
Dmax 17.0 nm
VolumePorod 429 nm3