KAP1 is an antiparallel dimer with a functional asymmetry.

Fonti G, Marcaida MJ, Bryan LC, Träger S, Kalantzi AS, Helleboid PJ, Demurtas D, Tully MD, Grudinin S, Trono D, Fierz B, Dal Peraro M, Life Sci Alliance 2(4) (2019) Europe PMC

SASDEW6 – KRAB-associated protein 1, (KAP1); TRIM28; 23-418 RBCC domain

Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain
MWexperimental 112 kDa
MWexpected 92 kDa
VPorod 381 nm3
log I(s) 3.90×101 3.90×100 3.90×10-1 3.90×10-2
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain small angle scattering data  s, nm-1
ln I(s)
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain Guinier plot ln 3.90×101 Rg: 8.3 nm 0 (8.3 nm)-2 s2
(sRg)2I(s)/I(0)
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain Kratky plot 1.104 0 √3 sRg
p(r)
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain pair distance distribution function Rg: 8.9 nm 0 Dmax: 37 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain GASBOR model
log I(s)
 s, nm-1
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain OTHER model
Synchrotron SAXS data from solutions of KRAB-associated protein 1, (KAP1); TRIM28; 23-418 RBCC (RING Finger-B Box-Coiled Coil) in 20 mM HEPES, 500 mM NaCl, 10 % v/v glycerol, 2 mM TCEP, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 12.00 mg/ml was measured at 20°C for 1 second. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Number of frames = UNKNOWN. SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN

Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain (RBCC DOMAIN KAP1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   46.1 kDa
 
UniProt   Q13263 (23-418)
Sequence   FASTA