Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins.

Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S, Biochem Biophys Res Commun 550:43-48 (2021) Europe PMC

SASDKE2 – Two titin I27 domains linked with AAE linker

Two titin I27 domains linked with AAE linker
MWexperimental 24 kDa
MWexpected 24 kDa
log I(s) 4.16×10-2 4.16×10-3 4.16×10-4 4.16×10-5
Two titin I27 domains linked with AAE linker small angle scattering data  s, nm-1
ln I(s)
Two titin I27 domains linked with AAE linker Guinier plot ln 4.16×10-2 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Two titin I27 domains linked with AAE linker Kratky plot 1.104 0 √3 sRg

Data validation

Fits and models

log I(s)
 s, nm-1
Two titin I27 domains linked with AAE linker Rg histogram Rg, nm
Synchrotron SAXS data from solutions of Two titin I27 domains linked with AAE linker in 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH 7.6 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 40 successive 0.900 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

SAXS data collected on two I27 domains linked by AAE linker

Two titin I27 domains linked with AAE linker (I27-(AAE)-RS)
Mol. type   Protein
Olig. state   Monomer
Mon. MW   23.8 kDa
 
UniProt   Q8WZ42
Sequence   FASTA