Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDF28 – Myotilin immunoglobulin domains Ig1Ig2 (220-452)

UniProt ID: Q9UBF9 (220-452) Myotilin Ig1Ig2 (220-452)

Myotilin Ig1Ig2 (220-452) experimental SAS data

Sample:	Myotilin Ig1Ig2 (220-452) monomer, 27 kDa Homo sapiens protein
Buffer:	20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2010 Nov 16

Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L

R_g^Guinier	3.7	nm
D_max	15.1	nm
Volume^Porod	52	nm³

SASDF38 – Myotilin immunoglobulin domains Ig1Ig2 (250-444)

UniProt ID: Q9UBF9 (250-444) Myotilin Ig1Ig2 (250-444)

Myotilin Ig1Ig2 (250-444) experimental SAS data

Sample:	Myotilin Ig1Ig2 (250-444) monomer, 22 kDa Homo sapiens protein
Buffer:	20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2019 Jun 27

R_g^Guinier	2.8	nm
D_max	10.1	nm
Volume^Porod	29	nm³

SASDF48 – Myotilin immunoglobulin domains Ig1Ig2 (250-498)

UniProt ID: Q9UBF9 (250-498) Myotilin Ig1Ig2 (250-498)

Myotilin Ig1Ig2 (250-498) experimental SAS data

Sample:	Myotilin Ig1Ig2 (250-498) monomer, 28 kDa Homo sapiens protein
Buffer:	20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2019 Jun 27

R_g^Guinier	3.2	nm
D_max	14.0	nm
Volume^Porod	37	nm³

SASDF58 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 10mM NaCl

UniProt ID: P12464 (1-173) DNA-directed RNA polymerase subunit delta

DNA-directed RNA polymerase subunit delta experimental SAS data

DNA-directed RNA polymerase subunit delta Kratky plot

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zidek L

R_g^Guinier	3.5	nm
D_max	14.0	nm
Volume^Porod	38	nm³

SASDF68 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 200 mM NaCl

UniProt ID: P12464 (1-173) DNA-directed RNA polymerase subunit delta

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	3.9	nm
D_max	20.0	nm
Volume^Porod	56	nm³

SASDF78 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 400 mM NaCl

UniProt ID: P12464 (1-173) DNA-directed RNA polymerase subunit delta

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.2	nm
D_max	20.0	nm
Volume^Porod	63	nm³

SASDF88 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 800 mM NaCl

UniProt ID: P12464 (1-173) DNA-directed RNA polymerase subunit delta

Sample:	DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.5	nm
D_max	21.0	nm
Volume^Porod	77	nm³

SASDF98 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 10mM NaCl

UniProt ID: P12464 (1-173) DNA-directed RNA polymerase subunit delta - mutant

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data

DNA-directed RNA polymerase subunit delta - mutant Kratky plot

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.3	nm
D_max	19.5	nm
Volume^Porod	58	nm³

SASDFA8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 200mM NaCl

UniProt ID: P12464 (1-173) DNA-directed RNA polymerase subunit delta - mutant

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.6	nm
D_max	22.0	nm
Volume^Porod	76	nm³

SASDFB8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 400mM NaCl

UniProt ID: P12464 (1-173) DNA-directed RNA polymerase subunit delta - mutant

Sample:	DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer:	20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3

R_g^Guinier	4.5	nm
D_max	24.0	nm
Volume^Porod	78	nm³

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