Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID: O60828

SASDED2 – Polyglutamine tract-binding protein 1 (PQBP-1)

UniProt ID: O60828 (1-265) Polyglutamine-binding protein 1

Polyglutamine-binding protein 1 experimental SAS data

Polyglutamine tract-binding protein 1 (PQBP-1) Rg histogram

Sample:	Polyglutamine-binding protein 1 monomer, 31 kDa Homo sapiens protein
Buffer:	20 mM Tris, 150 mM NaCl, 1mM DTT,, pH: 7
Experiment:	SAXS data collected at EMBL X33, DORIS III on 2009 Nov 18

Solution model of the intrinsically disordered polyglutamine tract-binding protein-1. Biophys J 102(7):1608-16 (2012)
Rees M, Gorba C, de Chiara C, Bui TT, Garcia-Maya M, Drake AF, Okazawa H, Pastore A, Svergun D, Chen YW

R_g^Guinier	3.7	nm
D_max	13.0	nm
Volume^Porod	51	nm³

SASDET6 – Polyglutamine-binding protein 1 p.Lys192Serfs7 (PQBP-1 XLID mutant K192Sfs7)

UniProt ID: O60828 (1-192) Polyglutamine-binding protein 1 p.Lys192Serfs*7

Polyglutamine-binding protein 1 p.Lys192Serfs*7 experimental SAS data

Polyglutamine-binding protein 1 p.Lys192Serfs*7 (PQBP-1 XLID mutant K192Sfs*7) Rg histogram

Sample:	Polyglutamine-binding protein 1 p.Lys192Serfs7 dimer, 47 kDa Homo sapiens* protein
Buffer:	Phosphate-buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL X33, DORIS III on 2013 Feb 15

Frameshift PQBP-1 mutants K192S^fs*7 and R153S^fs*41 implicated in X-linked intellectual disability form stable dimers. J Struct Biol (2019)
Rahman SK, Okazawa H, Chen YW

R_g^Guinier	3.5	nm
D_max	13.0	nm
Volume^Porod	114	nm³

SASDEU6 – Polyglutamine-binding protein 1 p.Arg153Serfs41 (PQBP-1 XLID mutant R153Sfs41)

UniProt ID: O60828 (1-154) Polyglutamine-binding protein 1 p.Arg153Serfs*41

Polyglutamine-binding protein 1 p.Arg153Serfs*41 experimental SAS data

Polyglutamine-binding protein 1 p.Arg153Serfs*41 (PQBP-1 XLID mutant R153Sfs*41) Rg histogram

Sample:	Polyglutamine-binding protein 1 p.Arg153Serfs41 dimer, 44 kDa Homo sapiens* protein
Buffer:	Phosphate-buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL X33, DORIS III on 2013 Feb 15