SASBDB entries for UniProt ID: P01308

SASDE25 – Human insulin hexamer from in-house SEC-SAXS

UniProt ID: P01308 (25-110) Human insulin
Human insulin experimental SAS data
DAMFILT model
Sample: Human insulin hexamer, 35 kDa Homo sapiens protein
Buffer: 7 mM sodium phosphate, 60 mM phenol, 200 µM Zn(CH3COO)2, 23 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 26
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.0 nm
Dmax 5.9 nm
VolumePorod 39 nm3

SASDJY3 – Immunoglobulin HUI-018 Fab in complex with human insulin

UniProt ID: P01308 (25-110) Insulin (Insulin B chain and Insulin A chain)

UniProt ID: None (None-None) HUI-018 Fab
Insulin (Insulin B chain and Insulin A chain)HUI-018 Fab experimental SAS data
OTHER model
Sample: Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Homo sapiens protein
HUI-018 Fab monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Mar 7
Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
RgGuinier 3.4 nm
Dmax 10.7 nm
VolumePorod 86 nm3

SASDJZ3 – Immunoglobulin HUI-018 Fab in complex with human insulin at high concentration

UniProt ID: P01308 (25-110) Insulin (Insulin B chain and Insulin A chain)

UniProt ID: None (None-None) HUI-018 Fab
Insulin (Insulin B chain and Insulin A chain)HUI-018 Fab experimental SAS data
OTHER model
Sample: Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Homo sapiens protein
HUI-018 Fab monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Mar 7
Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
RgGuinier 3.7 nm
Dmax 13.4 nm
VolumePorod 105 nm3

SASDEV5 – Insulin detemir tri-hexamer

UniProt ID: P01308 (25-110) Insulin detemir
Insulin detemir experimental SAS data
SASREF CV model
Sample: Insulin detemir 18-mer, 106 kDa protein
Buffer: 5.0 mM Na2HPO4, 13.1 mM m-cresol, 15.1 mM phenol, 173.7 mM glycerol, 20.0 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 3.3 nm
Dmax 11.3 nm
VolumePorod 137 nm3

SASDEW5 – Albumin-insulin detemir 1:6 complex, binding in Südlow's Site II

UniProt ID: P01308 (25-110) Insulin detemir (Levemir(R), Novo Nordisk A/S)

UniProt ID: P02768 (25-609) Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.)
Insulin detemir (Levemir(R), Novo Nordisk A/S)Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) experimental SAS data
SASREF CV model
Sample: Insulin detemir (Levemir(R), Novo Nordisk A/S) hexamer, 35 kDa protein
Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein
Buffer: 6.9 mM Na2HPO4, 11.9 mM m-cresol, 13.7 mM phenol, 157.3 mM glycerol, 38.5 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 162 nm3

SASDEX5 – Albumin-insulin detemir 1:6 complex, binding in Südlow's Site I

UniProt ID: P01308 (25-110) Insulin detemir (Levemir(R), Novo Nordisk A/S)

UniProt ID: P02768 (25-609) Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.)
Insulin detemir (Levemir(R), Novo Nordisk A/S)Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) experimental SAS data
SASREF CV model
Sample: Insulin detemir (Levemir(R), Novo Nordisk A/S) hexamer, 35 kDa protein
Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein
Buffer: 6.9 mM Na2HPO4, 11.9 mM m-cresol, 13.7 mM phenol, 157.3 mM glycerol, 38.5 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 162 nm3

SASDEZ5 – Albumin-insulin detemir 2:12 complex, P2 symmetry

UniProt ID: P02768 (25-609) Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.)

UniProt ID: P01308 (None-None) Insulin detemir (Levemir(R), Novo Nordisk A/S)
Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.)Insulin detemir (Levemir(R), Novo Nordisk A/S) experimental SAS data
SASREF CV model
Sample: Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein
Insulin detemir (Levemir(R), Novo Nordisk A/S) dodecamer, 71 kDa protein
Buffer: 8.8 mM Na2HPO4, 10.6 mM m-cresol, 12.2 mM phenol, 140.9 mM glycerol, 56.9 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 5.4 nm
Dmax 19.5 nm
VolumePorod 309 nm3

SASDEY5 – Albumin-insulin detemir 2:12 complex, P1 symmetry

UniProt ID: P02768 (25-609) Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.)

UniProt ID: P01308 (None-None) Insulin detemir (Levemir(R), Novo Nordisk A/S)
Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.)Insulin detemir (Levemir(R), Novo Nordisk A/S) experimental SAS data
SASREF CV model
Sample: Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein
Insulin detemir (Levemir(R), Novo Nordisk A/S) dodecamer, 71 kDa protein
Buffer: 8.8 mM Na2HPO4, 10.6 mM m-cresol, 12.2 mM phenol, 140.9 mM glycerol, 56.9 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 5.4 nm
Dmax 19.5 nm
VolumePorod 309 nm3

SASDE26 – Albumin-insulin degludec 1:12 complex

UniProt ID: P02768 (25-609) Human Albumin (Recombumin(R) Elite, Albumedix Ltd.)

UniProt ID: P01308 (25-110) Insulin degludec(Tresiba(R), Novo Nordisk A/S)
Human Albumin (Recombumin(R) Elite, Albumedix Ltd.)Insulin degludec(Tresiba(R), Novo Nordisk A/S) experimental SAS data
SASREF CV model
Sample: Human Albumin (Recombumin(R) Elite, Albumedix Ltd.) monomer, 66 kDa protein
Insulin degludec(Tresiba(R), Novo Nordisk A/S) dodecamer, 73 kDa protein
Buffer: 25 mM Na2HPO4, 15.9 mM m-cresol, 15.9 mM phenol, 212.8 mM glycerol, 20 mM NaCl, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 27
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 3.8 nm
Dmax 13.4 nm
VolumePorod 179 nm3