SASBDB entries for UniProt ID: P0DP23

SASDJ64 – Calcium bound Calmodulin

UniProt ID: P0DP23 (1-149) Calmodulin-1
Calmodulin-1 experimental SAS data
DENSS model
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Sep 24
A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 27 nm3

SASDJ74 – P454 peptide from B.pertussis CyaA toxin complexed with calmodulin

UniProt ID: P0DP23 (1-149) Calmodulin-1

UniProt ID: P0DKX7 (454-484) Bifunctional hemolysin/adenylate cyclase
Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data
DENSS model
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Bifunctional hemolysin/adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer: 20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Sep 24
A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A
RgGuinier 2.0 nm
Dmax 7.0 nm
VolumePorod 28 nm3

SASDNX3 – Calcium-bound Calmodulin, including structural models

UniProt ID: P0DP23 (2-149) Calmodulin-1
Calmodulin-1 experimental SAS data
Calcium-bound Calmodulin, including structural models Rg histogram
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Nov 15
Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium. BMC Biol 20(1):176 (2022)
Léger C, Pitard I, Sadi M, Carvalho N, Brier S, Mechaly A, Raoux-Barbot D, Davi M, Hoos S, Weber P, Vachette P, Durand D, Haouz A, Guijarro JI, Ladant D, Chenal A
RgGuinier 2.2 nm
Dmax 7.2 nm
VolumePorod 33 nm3

SASDNY3 – Calcium-bound Calmodulin complexed with Calmidazolium

UniProt ID: P0DP23 (2-149) Calmodulin-1

UniProt ID: None (None-None) Calmidazolium
Calmodulin-1Calmidazolium experimental SAS data
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Calmidazolium monomer, 1 kDa
Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Nov 15
Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium. BMC Biol 20(1):176 (2022)
Léger C, Pitard I, Sadi M, Carvalho N, Brier S, Mechaly A, Raoux-Barbot D, Davi M, Hoos S, Weber P, Vachette P, Durand D, Haouz A, Guijarro JI, Ladant D, Chenal A
RgGuinier 1.7 nm
Dmax 5.2 nm
VolumePorod 30 nm3

SASDPV6 – Calmodulin

UniProt ID: P0DP23 (1-149) Calmodulin-1
Calmodulin-1 experimental SAS data
Calmodulin-1 Kratky plot
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Sep 23
Binding by calmodulin is coupled to transient unfolding of the third FF domain of Prp40A. Protein Sci 32(4):e4606 (2023)
Díaz Casas A, Cordoba JJ, Ferrer BJ, Balakrishnan S, Wurm JE, Pastrana-Ríos B, Chazin WJ
RgGuinier 2.2 nm
Dmax 7.0 nm
VolumePorod 38 nm3

SASDPX6 – Calmodulin bound to the FF3 domain of pre-mRNA-processing factor 40 homolog A (Prp40A)

UniProt ID: P0DP23 (1-149) Calmodulin-1

UniProt ID: O75400 (516-592) Pre-mRNA-processing factor 40 homolog A
Calmodulin-1Pre-mRNA-processing factor 40 homolog A experimental SAS data
MODELLER model
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Pre-mRNA-processing factor 40 homolog A monomer, 9 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Sep 23
Binding by calmodulin is coupled to transient unfolding of the third FF domain of Prp40A. Protein Sci 32(4):e4606 (2023)
Díaz Casas A, Cordoba JJ, Ferrer BJ, Balakrishnan S, Wurm JE, Pastrana-Ríos B, Chazin WJ
RgGuinier 2.4 nm
Dmax 8.7 nm
VolumePorod 29 nm3

SASDPD4 – Ca2+-calmodulin

UniProt ID: P0DP23 (1-149) Calmodulin-1
Calmodulin-1 experimental SAS data
Calmodulin-1 Kratky plot
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4
New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H
RgGuinier 2.1 nm
Dmax 6.9 nm
VolumePorod 23 nm3

SASDPF4 – P2X7 ballast domain in complex with Ca2+-calmodulin

UniProt ID: P0DP23 (1-149) Calmodulin-1

UniProt ID: Q99572 (395-595) P2X purinoceptor 7
Calmodulin-1P2X purinoceptor 7 experimental SAS data
Calmodulin-1 P2X purinoceptor 7 Kratky plot
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
P2X purinoceptor 7 monomer, 25 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4
New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H
RgGuinier 3.2 nm
Dmax 13.5 nm
VolumePorod 73 nm3