SASBDB entries for UniProt ID: P0DTC9

SASDR83 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA Stem loop 2 and 3 of SARS-CoV-2 in HEPES conditions

UniProt ID: None (None-None) Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2

UniProt ID: P0DTC9 (44-180) Nucleoprotein
Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2Nucleoprotein experimental SAS data
Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 Nucleoprotein Kratky plot
Sample: Stem loop 2 and 3 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Nucleoprotein dimer, 30 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements
Karthikeyan Dhamotharan
RgGuinier 2.9 nm
Dmax 10.5 nm
VolumePorod 69 nm3

SASDR93 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA Stem loop 4 with AU extension of SARS-CoV-2 in HEPES conditions

UniProt ID: P0DTC9 (44-180) Nucleoprotein

UniProt ID: None (None-None) Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2
NucleoproteinStem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Stem loop 4 with AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements
Karthikeyan Dhamotharan
RgGuinier 3.2 nm
Dmax 11.8 nm
VolumePorod 50 nm3

SASDRA3 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA AU extension of SARS-CoV-2 in HEPES conditions

UniProt ID: None (None-None) AU extension in the 5'-genomic end of SARS-CoV-2

UniProt ID: P0DTC9 (44-180) Nucleoprotein
AU extension in the 5'-genomic end of SARS-CoV-2Nucleoprotein experimental SAS data
AU extension in the 5'-genomic end of SARS-CoV-2 Nucleoprotein Kratky plot
Sample: AU extension in the 5'-genomic end of SARS-CoV-2 monomer, 7 kDa Severe acute respiratory … RNA
Nucleoprotein dimer, 30 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements
Karthikeyan Dhamotharan
RgGuinier 2.6 nm
Dmax 9.0 nm
VolumePorod 46 nm3

SASDRB3 – N-terminal RNA-binding domain (NTD) of nucleocapsid protein (N) complexed with 5'-genomic RNA Stem loop 4 of SARS-CoV-2 in HEPES conditions

UniProt ID: P0DTC9 (44-180) Nucleoprotein

UniProt ID: None (None-None) Stem loop 4 in the 5'-genomic end of SARS-CoV-2
NucleoproteinStem loop 4 in the 5'-genomic end of SARS-CoV-2 experimental SAS data
Nucleoprotein Stem loop 4 in the 5'-genomic end of SARS-CoV-2 Kratky plot
Sample: Nucleoprotein monomer, 15 kDa Severe acute respiratory … protein
Stem loop 4 in the 5'-genomic end of SARS-CoV-2 monomer, 14 kDa Severe acute respiratory … RNA
Buffer: 25 mM HEPES, 75 mM KCl, 2.5 mM NaNO3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Nov 29
The preference signature of the SARS-CoV-2 Nucleocapsid NTD for its 5'-genomic RNA elements
Karthikeyan Dhamotharan
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 46 nm3

SASDXX5 – SARS-CoV-2 Nucleocapsid protein 0.5 mg/mL

UniProt ID: P0DTC9 (2-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.8 nm
Dmax 17.1 nm

SASDXY5 – SARS-CoV-2 Nucleocapsid protein 1 mg/mL

UniProt ID: P0DTC9 (2-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.6 nm
Dmax 18.8 nm

SASDXZ5 – SARS-CoV-2 Nucleocapsid protein 1.5 mg/mL

UniProt ID: P0DTC9 (2-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.7 nm
Dmax 18.8 nm

SASDX26 – SARS-CoV-2 Nucleocapsid protein 3 mg/mL

UniProt ID: P0DTC9 (2-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Nov 28
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 6.3 nm
Dmax 23.7 nm

SASDX36 – SARS-CoV-2 Nucleocapsid protein 4 mg/mL

UniProt ID: P0DTC9 (2-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Nov 28
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 6.6 nm
Dmax 22.5 nm

SASDX46 – Phosphorylated SARS-CoV-2 Nucleocapsid protein 0.5 mg/mL

UniProt ID: P0DTC9 (2-419) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 95 kDa Severe acute respiratory … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2023 Jul 18
Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states. Nat Commun 16(1):7970 (2025)
Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
RgGuinier 5.6 nm
Dmax 15.0 nm