SASBDB entries for UniProt ID: P0DTC9

SASDSS9 – SARS-CoV-2 N-protein (N1-245, residues 1-245): 76.4 µM

UniProt ID: P0DTC9 (1-245) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 55 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 4.0 nm
Dmax 18.0 nm
VolumePorod 67 nm3

SASDSU9 – SARS-CoV-2 N-protein (N1-245, residues 1-245): 400 µM

UniProt ID: P0DTC9 (1-245) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein tetramer, 110 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 5.4 nm
Dmax 24.0 nm
VolumePorod 157 nm3

SASDSV9 – SARS-CoV-2 N-protein (N1-245; residues 1-245) L223P, L227P and L230P triple mutant: 36.4 µM

UniProt ID: P0DTC9 (1-245) Nucleoprotein (L223P, L227P, L230P)
Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 3.2 nm
Dmax 13.5 nm
VolumePorod 53 nm3

SASDSW9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 72.7 µM

UniProt ID: P0DTC9 (1-245) Nucleoprotein (L223P, L227P, L230P)
Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 3.5 nm
Dmax 14.5 nm
VolumePorod 47 nm3

SASDVU6 – SARS-CoV-2 N-protein (N1-245; amino acids 1-245): 167 µM

UniProt ID: P0DTC9 (1-245) Nucleoprotein
Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 55 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 5.2 nm
Dmax 20.5 nm
VolumePorod 121 nm3

SASDSX9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 294 µM

UniProt ID: P0DTC9 (1-245) Nucleoprotein (L223P, L227P, L230P)
Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 4.4 nm
Dmax 19.0 nm
VolumePorod 68 nm3

SASDSY9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 400 µM

UniProt ID: P0DTC9 (1-245) Nucleoprotein (L223P, L227P, L230P)
Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Jan 29
Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN
RgGuinier 4.6 nm
Dmax 19.0 nm
VolumePorod 75 nm3