SASBDB entries for UniProt ID: P10205

SASDKW8 – Full-length pUL21 with C-terminal His-tag

UniProt ID: P10205 (1-535) Tegument protein UL21
Tegument protein UL21 experimental SAS data
Full-length pUL21 with C-terminal His-tag Rg histogram
Sample: Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread. PLoS Pathog 17(8):e1009824 (2021)
Benedyk TH, Muenzner J, Connor V, Han Y, Brown K, Wijesinghe KJ, Zhuang Y, Colaco S, Stoll GA, Tutt OS, Svobodova S, Svergun DI, Bryant NA, Deane JE, Firth AE, Jeffries CM, Crump CM, Graham SC
RgGuinier 4.2 nm
Dmax 18.1 nm
VolumePorod 90 nm3

SASDNB7 – C-terminal domain of pUL21 with N-terminal His-tag

UniProt ID: P10205 (275-535) Tegument protein UL21 (C-terminal domain)
Tegument protein UL21 (C-terminal domain) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 2.2 nm
Dmax 8.5 nm
VolumePorod 49 nm3

SASDND7 – Complex of H6-pUL21C and H6-miniCERTL

UniProt ID: P10205 (275-535) Tegument protein UL21 (C-terminal domain)

UniProt ID: Q9Y5P4 (None-None) Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624)
Tegument protein UL21 (C-terminal domain)Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 3.3 nm
Dmax 13.6 nm
VolumePorod 84 nm3

SASDNE7 – Complex of H6-miniCERTL and full-length pUL21-H6

UniProt ID: P10205 (1-535) Tegument protein UL21

UniProt ID: Q9Y5P4 (None-None) Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624)
Tegument protein UL21Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
RgGuinier 4.1 nm
Dmax 15.8 nm
VolumePorod 158 nm3