Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID: P11274

SASDC26 – DH-PH - Dbl-homology domain (DH) and Pleckstrin-homology (PH) of Bcr-Abl tyrosine kinase p210

UniProt ID: P11274 (None-None) BCR-ABL p210 fusion protein (DH-PH)

BCR-ABL p210 fusion protein (DH-PH) experimental SAS data

DH-PH - Dbl-homology domain (DH) and Pleckstrin-homology (PH) of Bcr-Abl tyrosine kinase p210 Rg histogram

Sample:	BCR-ABL p210 fusion protein (DH-PH) monomer, 47 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Oct 13

Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase. Nat Commun 8(1):2101 (2017)
Reckel S, Gehin C, Tardivon D, Georgeon S, Kükenshöner T, Löhr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Güntert P, Dötsch V, Koide S, Gavin AC, Hantschel O

R_g^Guinier	3.2	nm
D_max	11.1	nm
Volume^Porod	69	nm³

SASDC36 – DH - Dbl-homology domain of Bcr-Abl tyrosine kinase p210

UniProt ID: P11274 (None-None) BCR-ABL p210 fusion protein

BCR-ABL p210 fusion protein experimental SAS data

Sample:	BCR-ABL p210 fusion protein monomer, 25 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Oct 13

R_g^Guinier	2.1	nm
D_max	7.3	nm
Volume^Porod	38	nm³

SASDC46 – PH - Pleckstrin-homology domain of Bcr-Abl tyrosine kinase p210

UniProt ID: P11274 (None-None) BCR-ABL p210 fusion protein (PH domain)

BCR-ABL p210 fusion protein (PH domain) experimental SAS data

Sample:	BCR-ABL p210 fusion protein (PH domain) monomer, 22 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Nov 10

R_g^Guinier	2.0	nm
D_max	6.6	nm
Volume^Porod	38	nm³