SASBDB entries for UniProt ID: P16144

SASDAT6 – Integrin beta4, fragment of the cytoplasmic region encompassing the third and fourth FnIII domains

UniProt ID: P16144 (None-None) Integrin beta-4
Integrin beta-4 experimental SAS data
DAMMIF model
Sample: Integrin beta-4 monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 6
Combination of X-ray crystallography, SAXS and DEER to obtain the structure of the FnIII-3,4 domains of integrin α6β4. Acta Crystallogr D Biol Crystallogr 71(Pt 4):969-85 (2015)
Alonso-García N, García-Rubio I, Manso JA, Buey RM, Urien H, Sonnenberg A, Jeschke G, de Pereda JM
RgGuinier 2.2 nm
Dmax 7.0 nm
VolumePorod 32 nm3

SASDDE8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4)

UniProt ID: P16144-2 (1436-1666) Integrin beta-4 fragment with final part of the connecting segment and the third and fourth FnIII domains
Integrin beta-4 fragment with final part of the connecting segment and the third and fourth FnIII domains experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4) Rg histogram
Sample: Integrin beta-4 fragment with final part of the connecting segment and the third and fourth FnIII domains monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes. Structure 27(6):952-964.e6 (2019)
Manso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 37 nm3

SASDDF8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463E

UniProt ID: P16144-2 (1436-1666) Integrin beta-4 (1436-1666) R1463E
Integrin beta-4 (1436-1666) R1463E experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463E Rg histogram
Sample: Integrin beta-4 (1436-1666) R1463E monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes. Structure 27(6):952-964.e6 (2019)
Manso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDDG8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A

UniProt ID: P16144-2 (1436-1666) Integrin beta-4 (1436-1666) R1463A
Integrin beta-4 (1436-1666) R1463A experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A Rg histogram
Sample: Integrin beta-4 (1436-1666) R1463A monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes. Structure 27(6):952-964.e6 (2019)
Manso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 36 nm3

SASDDH8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468R

UniProt ID: P16144-2 (1436-1666) Integrin beta-4 (1436-1666) A1468R
Integrin beta-4 (1436-1666) A1468R experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468R Rg histogram
Sample: Integrin beta-4 (1436-1666) A1468R monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes. Structure 27(6):952-964.e6 (2019)
Manso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM
RgGuinier 2.8 nm
Dmax 10.9 nm
VolumePorod 35 nm3

SASDDJ8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468D

UniProt ID: P16144-2 (1436-1666) Integrin beta-4 (1436-1666) A1468D
Integrin beta-4 (1436-1666) A1468D experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468D Rg histogram
Sample: Integrin beta-4 (1436-1666) A1468D monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes. Structure 27(6):952-964.e6 (2019)
Manso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM
RgGuinier 2.7 nm
Dmax 11.0 nm
VolumePorod 34 nm3

SASDDK8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475E

UniProt ID: P16144-2 (1436-1666) Integrin beta-4 (1436-1666) R1475E
Integrin beta-4 (1436-1666) R1475E experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475E Rg histogram
Sample: Integrin beta-4 (1436-1666) R1475E monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes. Structure 27(6):952-964.e6 (2019)
Manso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM
RgGuinier 2.6 nm
Dmax 9.5 nm
VolumePorod 42 nm3

SASDDL8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475A

UniProt ID: P16144-2 (1436-1666) Integrin beta-4 (1436-1666) R1475A
Integrin beta-4 (1436-1666) R1475A experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475A Rg histogram
Sample: Integrin beta-4 (1436-1666) R1475A monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes. Structure 27(6):952-964.e6 (2019)
Manso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDDM8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542E

UniProt ID: P16144-2 (1436-1666) Integrin beta-4 (1436-1666) R1542E
Integrin beta-4 (1436-1666) R1542E experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542E Rg histogram
Sample: Integrin beta-4 (1436-1666) R1542E monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes. Structure 27(6):952-964.e6 (2019)
Manso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDDN8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542A

UniProt ID: P16144-2 (1436-1666) Integrin beta-4 (1436-1666) R1542A
Integrin beta-4 (1436-1666) R1542A experimental SAS data
Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542A Rg histogram
Sample: Integrin beta-4 (1436-1666) R1542A monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26
Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes. Structure 27(6):952-964.e6 (2019)
Manso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM
RgGuinier 2.3 nm
Dmax 8.5 nm
VolumePorod 34 nm3