SASBDB entries for UniProt ID: P69905

SASDNS2 – Experimental SAXS data for native hemoglobin (Hb) at concentration c = 5 mg/ml

UniProt ID: P69905 (3-142) Hemoglobin subunit alpha

UniProt ID: P68871 (3-147) Hemoglobin subunit beta
Hemoglobin subunit alphaHemoglobin subunit beta experimental SAS data
GASBOR model
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Buffer: Ringer's lactate solution, pH: 6.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19
Solution Structure of Poly(ethylene) Glycol-Conjugated Hemoglobin Revealed by Small-Angle X-Ray Scattering: Implications for a New Oxygen Therapeutic Biophysical Journal 94(1):173-181 (2008)
Svergun D, Ekström F, Vandegriff K, Malavalli A, Baker D, Nilsson C, Winslow R
RgGuinier 2.4 nm
Dmax 13.0 nm

SASDNT2 – Experimental SAXS data for native hemoglobin (Hb) at concentration c = 31.25 mg/ml

UniProt ID: P69905 (3-142) Hemoglobin subunit alpha

UniProt ID: P68871 (3-147) Hemoglobin subunit beta
Hemoglobin subunit alphaHemoglobin subunit beta experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Kratky plot
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Buffer: Ringer's lactate solution, pH: 6.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19
Solution Structure of Poly(ethylene) Glycol-Conjugated Hemoglobin Revealed by Small-Angle X-Ray Scattering: Implications for a New Oxygen Therapeutic Biophysical Journal 94(1):173-181 (2008)
Svergun D, Ekström F, Vandegriff K, Malavalli A, Baker D, Nilsson C, Winslow R
RgGuinier 2.2 nm

SASDRN8 – α/β Hemoglobin (human)

UniProt ID: P69905 (1-142) Hemoglobin subunit alpha

UniProt ID: P68871 (1-147) Hemoglobin subunit beta
Hemoglobin subunit alphaHemoglobin subunit beta experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Kratky plot
Sample: Hemoglobin subunit alpha dimer, 31 kDa Homo sapiens protein
Hemoglobin subunit beta dimer, 32 kDa Homo sapiens protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2021 Oct 7
NSRRC TPS13A standard protein archive
Orion Shih
RgGuinier 2.5 nm
Dmax 9.2 nm
VolumePorod 97 nm3

SASDV62 – Human Haemoglobin (Sigma-Aldrich) in 100mM Sodium Phosphate buffer, pH=7.0, SEC-SAXS

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100 mM Sodium Phosphate, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 May 24
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 6.8 nm
VolumePorod 105 nm3

SASDV72 – Human Haemoglobin (Sigma-Aldrich) in 100mM Sodium Phosphate buffer, pH=7.0, c=5mg/ml, 1 day after SEC

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100 mM Sodium Phosphate, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Sep 30
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 6.7 nm
VolumePorod 103 nm3

SASDV82 – Human Haemoglobin (Sigma-Aldrich) with 5% (w/v) PEG600 in 100mM Sodium Phosphate buffer pH=7.0, c=5 mg/ml

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100mM Sodium Phosphate buffer with 5% (w/v) PEG600, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Sep 30
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 6.7 nm
VolumePorod 105 nm3

SASDV92 – Human Haemoglobin (Sigma-Aldrich) with 10% (w/v) PEG600 in 100mM Sodium Phosphate buffer pH=7.0, c=5 mg/ml

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100mM Sodium Phosphate buffer with 10% (w/v) PEG600, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Sep 30
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 7.2 nm
VolumePorod 113 nm3

SASDVA2 – Human Haemoglobin (Sigma-Aldrich) with 20% (w/v) PEG600 in 100mM Sodium Phosphate buffer pH=7.0, c=5 mg/ml

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100mM Sodium Phosphate buffer with 20% (w/v) PEG600, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Sep 30
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.6 nm
Dmax 7.6 nm
VolumePorod 113 nm3

SASDVB2 – Human Haemoglobin (Sigma-Aldrich) in 100mM Sodium Phosphate buffer, pH=7.0, c=5mg/ml, 1 week after SEC

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit alphaHemoglobin subunit betaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit alpha Hemoglobin subunit beta Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100mM Sodium Phosphate buffer with 5% (w/v) PEG2000, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Oct 6
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 6.8 nm
VolumePorod 107 nm3

SASDVC2 – Human Haemoglobin (Sigma-Aldrich) with 5% PEG2000 in 100mM Sodium Phosphate buffer, pH=7.0, c=5mg/ml

UniProt ID: P68871 (2-147) Hemoglobin subunit beta

UniProt ID: P69905 (2-142) Hemoglobin subunit alpha

UniProt ID: None (None-None) Protoporphyrin IX containing fe
Hemoglobin subunit betaHemoglobin subunit alphaProtoporphyrin IX containing fe experimental SAS data
Hemoglobin subunit beta Hemoglobin subunit alpha Protoporphyrin IX containing fe Kratky plot
Sample: Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein
Protoporphyrin IX containing fe monomer, 1 kDa
Buffer: 100mM Sodium Phosphate buffer with 5% (w/v) PEG2000, pH: 7
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Oct 6
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications The Journal of Physical Chemistry B (2024)
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B
RgGuinier 2.4 nm
Dmax 6.5 nm
VolumePorod 92 nm3