SASBDB entries for UniProt ID: P9WMU1

SASDFZ2 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 315-319)

UniProt ID: P9WMU1 (None-None) Cell wall synthesis protein Wag31
Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 12.1 nm

SASDF23 – Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 320-324)

UniProt ID: P9WMU1 (None-None) Cell wall synthesis protein Wag31
Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31, 30 kDa Mycobacterium tuberculosis protein
Buffer: 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 29
Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31. J Struct Biol :107429 (2019)
Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B
RgGuinier 10.2 nm

SASDHH4 – N-terminal domain of Wag31 from M. tuberculosis

UniProt ID: P9WMU1 (2-60) Cell wall synthesis protein Wag31
Cell wall synthesis protein Wag31 experimental SAS data
Cell wall synthesis protein Wag31 Kratky plot
Sample: Cell wall synthesis protein Wag31 tetramer, 34 kDa Mycobacterium tuberculosis protein
Buffer: 20 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Sep 3
Structural basis of self-assembly in the lipid-binding domain of mycobacterial polar growth factor Wag31 IUCrJ 7(4):767-776 (2020)
Choukate K, Chaudhuri B
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 52 nm3