Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID: Q15185

SASDBH6 – Full-length human p23 (1-160)

UniProt ID: Q15185 (1-160) Prostaglandin E synthase 3

Prostaglandin E synthase 3 experimental SAS data

Prostaglandin E synthase 3 Kratky plot

Sample:	Prostaglandin E synthase 3 monomer, 19 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Jun 22

The C-terminal region of the human p23 chaperone modulates its structure and function. Arch Biochem Biophys 565:57-67 (2015)
Seraphim TV, Gava LM, Mokry DZ, Cagliari TC, Barbosa LR, Ramos CH, Borges JC

R_g^Guinier	2.5	nm
D_max	10.0	nm
Volume^Porod	40	nm³

SASDBJ6 – Truncated construct of human p23 (1-142)

UniProt ID: Q15185 (1-142) Prostaglandin E synthase 3 (1-142)

Prostaglandin E synthase 3 (1-142) experimental SAS data

Prostaglandin E synthase 3 (1-142) Kratky plot

Sample:	Prostaglandin E synthase 3 (1-142) monomer, 17 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Jun 22

The C-terminal region of the human p23 chaperone modulates its structure and function. Arch Biochem Biophys 565:57-67 (2015)
Seraphim TV, Gava LM, Mokry DZ, Cagliari TC, Barbosa LR, Ramos CH, Borges JC

R_g^Guinier	2.1	nm
D_max	8.5	nm
Volume^Porod	36	nm³

SASDBL6 – Truncated construct of human p23 (1-117)

UniProt ID: Q15185 (1-117) Prostaglandin E synthase 3 (1-117)

Prostaglandin E synthase 3 (1-117) experimental SAS data

Prostaglandin E synthase 3 (1-117) Kratky plot

Sample:	Prostaglandin E synthase 3 (1-117) monomer, 14 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2013 Jun 21

The C-terminal region of the human p23 chaperone modulates its structure and function. Arch Biochem Biophys 565:57-67 (2015)
Seraphim TV, Gava LM, Mokry DZ, Cagliari TC, Barbosa LR, Ramos CH, Borges JC

R_g^Guinier	1.9	nm
D_max	7.0	nm
Volume^Porod	29	nm³

SASDBK6 – Truncated construct of human p23 (1-131)

UniProt ID: Q15185 (1-131) Prostaglandin E synthase 3 (1-131)

Prostaglandin E synthase 3 (1-131) experimental SAS data

Prostaglandin E synthase 3 (1-131) Kratky plot

Sample:	Prostaglandin E synthase 3 (1-131) monomer, 16 kDa Homo sapiens protein
Buffer:	25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2013 Jun 21

The C-terminal region of the human p23 chaperone modulates its structure and function. Arch Biochem Biophys 565:57-67 (2015)
Seraphim TV, Gava LM, Mokry DZ, Cagliari TC, Barbosa LR, Ramos CH, Borges JC

R_g^Guinier	1.9	nm
D_max	7.0	nm
Volume^Porod	31	nm³