SASBDB entries for UniProt ID: Q16595

SASDDD3 – Human mitochondrial cysteine desulfurase-ISCU-Frataxin (NFS1, ISD11 and Acp heterodimer complex)

UniProt ID: Q9Y697 (53-457) Cysteine desulfurase, mitochondrial

UniProt ID: Q9HD34 (None-None) LYR motif-containing protein 4

UniProt ID: P0A6A8 (None-None) Acyl carrier protein

UniProt ID: Q9H1K1 (33-167) Iron-sulfur cluster assembly enzyme ISCU, mitochondrial

UniProt ID: Q16595 (81-210) Frataxin, mitochondrial
Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier proteinIron-sulfur cluster assembly enzyme ISCU, mitochondrialFrataxin, mitochondrial experimental SAS data
HADDOCK model
Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein
LYR motif-containing protein 4 dimer, 23 kDa Homo sapiens protein
Acyl carrier protein dimer, 22 kDa Escherichia coli protein
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial dimer, 29 kDa Homo sapiens protein
Frataxin, mitochondrial dimer, 29 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 Apr 18
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure 26(8):1127-1136.e4 (2018)
Cai K, Frederick RO, Dashti H, Markley JL
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 287 nm3

SASDWT6 – Frataxin bound to tailored nanobody 16C10

UniProt ID: Q16595 (90-210) Frataxin, mitochondrial

UniProt ID: None (1-139) Nanobody 16C10
Frataxin, mitochondrialNanobody 16C10 experimental SAS data
COOT model
Sample: Frataxin, mitochondrial monomer, 14 kDa Homo sapiens protein
Nanobody 16C10 monomer, 15 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Sep 18
Nanobodies as tools for studying human frataxin biology. Commun Biol (2026)
Pignataro MF, Fernández NB, Garay-Alvarez A, Pavan MF, Molina R, Muñoz IG, Grossi J, Noguera M, Vila A, García AE, Gentili HG, Rodríguez NA, Aran M, Parreño V, Bok M, Hermoso JA, Ibañez LI, Santos J
RgGuinier 2.4 nm
Dmax 8.0 nm
VolumePorod 37 nm3

SASDWS6 – Frataxin bound to tailored nanobody 28F6

UniProt ID: Q16595 (90-210) Frataxin, mitochondrial

UniProt ID: None (1-142) Nanobody 28F6
Frataxin, mitochondrialNanobody 28F6 experimental SAS data
ALPHAFOLD model
Sample: Frataxin, mitochondrial monomer, 14 kDa Homo sapiens protein
Nanobody 28F6 monomer, 15 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Sep 18
Nanobodies as tools for studying human frataxin biology. Commun Biol (2026)
Pignataro MF, Fernández NB, Garay-Alvarez A, Pavan MF, Molina R, Muñoz IG, Grossi J, Noguera M, Vila A, García AE, Gentili HG, Rodríguez NA, Aran M, Parreño V, Bok M, Hermoso JA, Ibañez LI, Santos J
RgGuinier 2.2 nm
Dmax 7.4 nm
VolumePorod 36 nm3