Small Angle Scattering Biological Data Bank SASBDB

Browse by DISSEMINATION: Published

SASDLY8 – Mycobacterium tuberculosis Rv0792c, a GntR homolog

Recombinant Mycobacterium tuberculosis H37Rv experimental SAS data

Sample:	Recombinant Mycobacterium tuberculosis H37Rv dimer, 65 kDa Mycobacterium tuberculosis H37Rv protein
Buffer:	25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 2

Structural and Functional Characterization of Rv0792c from Mycobacterium tuberculosis: Identifying Small Molecule Inhibitor against HutC Protein. Microbiol Spectr :e0197322 (2022)
Chauhan NK, Anand A, Sharma A, Dhiman K, Gosain TP, Singh P, Singh P, Khan E, Chattopadhyay G, Kumar A, Sharma D, Ashish, Sharma TK, Singh R

R_g^Guinier	3.2	nm
D_max	12.0	nm

SASDLZ8 – SS DNA Aptamer 1 which binds Mtb GntR Homolog [Rv0792c_1]

SSDNA Aptamer from SELEX against Mtb Rv0792c experimental SAS data

Sample:	SSDNA Aptamer from SELEX against Mtb Rv0792c monomer, 13 kDa unidentified DNA
Buffer:	25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 20

R_g^Guinier	2.1	nm
D_max	8.6	nm

SASDMA9 – Diacetylchitobiose deacetylase (isolated hexamer from SEC-SAXS)

Diacetylchitobiose deacetylase experimental SAS data

Sample:	Diacetylchitobiose deacetylase hexamer, 186 kDa Thermococcus chitonophagus protein
Buffer:	20 mM TRIS, 200 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Sep 26

Structural, Thermodynamic and Enzymatic Characterization of N,N-Diacetylchitobiose Deacetylase from Pyrococcus chitonophagus. Int J Mol Sci 23(24) (2022)
Biniek-Antosiak K, Bejger M, Śliwiak J, Baranowski D, Mohammed ASA, Svergun DI, Rypniewski W

R_g^Guinier	3.6	nm
D_max	11.2	nm
Volume^Porod	317	nm³

SASDMB9 – Diacetylchitobiose deacetylase (oligomeric mixture of hexamers and dodecamers)

Sample:	Diacetylchitobiose deacetylase, 185 kDa Thermococcus chitonophagus protein
Buffer:	20 mM TRIS, 200 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Sep 26

R_g^Guinier

3.9

SASDPY2 – SARS-CoV-2 non-structural protein 7-8 (nsp7-8) polyprotein monomer

Replicase polyprotein 1ab experimental SAS data

Sample:	Replicase polyprotein 1ab monomer, 31 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15

Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E

R_g^Guinier	2.5	nm
D_max	8.8	nm
Volume^Porod	50	nm³

SASDPZ2 – SARS-CoV-2 non-structural protein 7-8 (nsp7-8) polyprotein dimer

Sample:	Replicase polyprotein 1ab dimer, 63 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15

R_g^Guinier	3.3	nm
D_max	11.8	nm
Volume^Porod	110	nm³

SASDP23 – SARS-CoV-2 non-structural protein 7-11 (nsp7-11) polyprotein monomer

Replicase polyprotein 1a experimental SAS data

Sample:	Replicase polyprotein 1a monomer, 60 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14

R_g^Guinier	3.5	nm
D_max	15.6	nm
Volume^Porod	102	nm³

SASDP33 – SARS-CoV-2 non-structural protein 7-11 (nsp7-11) polyprotein dimer

Sample:	Replicase polyprotein 1a dimer, 119 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14

R_g^Guinier	4.6	nm
D_max	19.1	nm
Volume^Porod	203	nm³

SASDMD7 – ESX-1 secretion-associated protein EspB large construct

ESX-1 secretion-associated protein EspB experimental SAS data

ESX-1 secretion-associated protein EspB Kratky plot

Sample:	ESX-1 secretion-associated protein EspB monomer, 48 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Sep 21

The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
Gijsbers A, Eymery M, Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG

R_g^Guinier	5.0	nm
D_max	19.2	nm
Volume^Porod	119	nm³

SASDME7 – ESX-1 secretion-associated protein EspB medium construct

Sample:	ESX-1 secretion-associated protein EspB monomer, 37 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Sep 21

R_g^Guinier	3.5	nm
D_max	13.3	nm
Volume^Porod	75	nm³

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