Browse by DISSEMINATION: Published

SASDMF7 – ESX-1 secretion-associated protein EspB small construct

ESX-1 secretion-associated protein EspB experimental SAS data
ESX-1 secretion-associated protein EspB Kratky plot
Sample: ESX-1 secretion-associated protein EspB monomer, 30 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 21
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
Gijsbers A, Eymery M, Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 3.3 nm
Dmax 10.8 nm
VolumePorod 56 nm3

SASDMG7 – ESX-1 secretion-associated protein EspB large construct bound to ESX-1 secretion-associated protein EspK — EspBL-K complex

ESX-1 secretion-associated protein EspKESX-1 secretion-associated protein EspB experimental SAS data
ESX-1 secretion-associated protein EspK ESX-1 secretion-associated protein EspB Kratky plot
Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein
ESX-1 secretion-associated protein EspB monomer, 48 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 12
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
Gijsbers A, Eymery M, Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 5.0 nm
Dmax 18.3 nm
VolumePorod 128 nm3

SASDMH7 – ESX-1 secretion-associated protein EspB medium construct bound to ESX-1 secretion-associated protein EspK — EspBM-K complex

ESX-1 secretion-associated protein EspKESX-1 secretion-associated protein EspB experimental SAS data
CORAL model
Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein
ESX-1 secretion-associated protein EspB monomer, 37 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 12
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
Gijsbers A, Eymery M, Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 4.3 nm
Dmax 15.7 nm
VolumePorod 100 nm3

SASDMJ7 – ESX-1 secretion-associated protein EspB small construct bound to ESX-1 secretion-associated protein EspK — EspBS-K complex

ESX-1 secretion-associated protein EspKESX-1 secretion-associated protein EspB experimental SAS data
ESX-1 secretion-associated protein EspK ESX-1 secretion-associated protein EspB Kratky plot
Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein
ESX-1 secretion-associated protein EspB monomer, 30 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 12
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
Gijsbers A, Eymery M, Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 4.1 nm
Dmax 13.0 nm
VolumePorod 113 nm3

SASDQF4 – ESX-1 secretion-associated protein EspB

ESX-1 secretion-associated protein EspB experimental SAS data
ESX-1 secretion-associated protein EspB Kratky plot
Sample: ESX-1 secretion-associated protein EspB heptamer, 261 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 12
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
Gijsbers A, Eymery M, Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 5.9 nm
Dmax 18.8 nm
VolumePorod 911 nm3

SASDMG9 – Multidrug resistance operon repressor (MexR) of the MexAB-OprM multidrug efflux pump operon of Pseudomonas aeruginosa

Multidrug resistance operon repressor experimental SAS data
DAMFILT model
Sample: Multidrug resistance operon repressor dimer, 32 kDa Pseudomonas aeruginosa protein
Buffer: 20mM HEPES, 150mM NaCl, 10mM DTT, 1% v/v glycerol, pH: 7.1
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Nov 23
Small-angle X-ray and neutron scattering of MexR and its complex with DNA supports a conformational selection binding model Biophysical Journal (2022)
Caporaletti F, Pietras Z, Morad V, Mårtensson L, Gabel F, Wallner B, Martel A, Sunnerhagen M
RgGuinier 2.3 nm
Dmax 7.7 nm
VolumePorod 56 nm3

SASDMH9 – Pseudomonas aeruginosa Multidrug resistance operon repressor (MexR) in complex with 34bp dsDNA binding sequence (SANS data at 0% D2O)

Multidrug resistance operon repressor34 base pair double-stranded DNA experimental SAS data
MONSA model
Sample: Multidrug resistance operon repressor dimer, 32 kDa Pseudomonas aeruginosa protein
34 base pair double-stranded DNA monomer, 21 kDa synthetic construct DNA
Buffer: 20mM NaPO4, 150 mM NaCl, 10 mM DTT, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 May 30
Small-angle X-ray and neutron scattering of MexR and its complex with DNA supports a conformational selection binding model Biophysical Journal (2022)
Caporaletti F, Pietras Z, Morad V, Mårtensson L, Gabel F, Wallner B, Martel A, Sunnerhagen M
RgGuinier 2.9 nm
Dmax 7.8 nm
VolumePorod 79 nm3

SASDLQ9 – Von Willebrand Factor C6 domain wildtype

von Willebrand factor C6 domain wild type experimental SAS data
von Willebrand factor C6 domain wild type Kratky plot
Sample: Von Willebrand factor C6 domain wild type monomer, 9 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 11
Structure and dynamics of the von Willebrand Factor C6 domain Journal of Structural Biology 214(4):107923 (2022)
Chen P, Kutzki F, Mojzisch A, Simon B, Xu E, Aponte-Santamaría C, Horny K, Jeffries C, Schneppenheim R, Wilmanns M, Brehm M, Gräter F, Hennig J
RgGuinier 1.7 nm
Dmax 6.6 nm
VolumePorod 12 nm3

SASDPY6 – BTB domain of longitudinals lacking protein (LOLA) at 1.0 mg/ml

Longitudinals lacking protein, isoform G experimental SAS data
ALPHAFOLD model
Sample: Longitudinals lacking protein, isoform G hexamer, 92 kDa Drosophila melanogaster protein
Buffer: 20 mM Tris, pH 7.4, 200 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 8
BTB domains: A structural view of evolution, multimerization, and protein-protein interactions. Bioessays :e2200179 (2022)
Bonchuk A, Balagurov K, Georgiev P
RgGuinier 4.1 nm
Dmax 20.0 nm
VolumePorod 213 nm3

SASDPZ6 – BTB domain of longitudinals lacking protein (LOLA) at 3.0 mg/ml

Longitudinals lacking protein, isoform G experimental SAS data
Longitudinals lacking protein, isoform G Kratky plot
Sample: Longitudinals lacking protein, isoform G hexamer, 92 kDa Drosophila melanogaster protein
Buffer: 20 mM Tris, pH 7.4, 200 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 8
BTB domains: A structural view of evolution, multimerization, and protein-protein interactions. Bioessays :e2200179 (2022)
Bonchuk A, Balagurov K, Georgiev P
RgGuinier 4.8 nm
Dmax 24.0 nm
VolumePorod 263 nm3