Browse by DISSEMINATION: Published

SASDQR7 – Bicelles formed by DMPC DMPG and DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholineDMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineDMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol experimental SAS data
DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol Kratky plot
Sample: DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M

SASDQS7 – Bicelles formed by POPC CHAPS

CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonatePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine experimental SAS data
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine Kratky plot
Sample: CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M

SASDQT7 – Bicelles formed by POPC cholate

Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acidPOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine experimental SAS data
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine Kratky plot
Sample: Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M

SASDQU7 – Bicelles formed by POPC DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholinePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine experimental SAS data
DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine Kratky plot
Sample: DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M

SASDQV7 – Bicelles formed by POPC POPG and CHAPS

CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonatePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholinePOPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) experimental SAS data
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) Kratky plot
Sample: CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M

SASDQW7 – Bicelles formed by POPC POPG and cholate

Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acidPOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholinePOPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) experimental SAS data
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) Kratky plot
Sample: Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M

SASDQX7 – Bicelles formed by POPC POPG and DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholinePOPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholinePOPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) experimental SAS data
DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) Kratky plot
Sample: DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid
POPC - 2-oleoyl-1-palmitoyl-sn-glyecro-3-phosphocholine None, lipid
POPG - 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M

SASDL66 – High mannose neurofascin 155 immunoglobulin domains 1-6, 37.2 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.7 nm
Dmax 18.0 nm
VolumePorod 355 nm3

SASDL76 – High mannose neurofascin 155 immunoglobulin domains 1-6, 73.5 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 5.0 nm
Dmax 21.0 nm
VolumePorod 450 nm3

SASDL86 – High mannose neurofascin 155 immunoglobulin domains 1-6, 19.7 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.5 nm
Dmax 17.0 nm
VolumePorod 290 nm3