Browse by DISSEMINATION: Published

SASDP97 – Bovine serum albumin at neutral pH, 50 °C, and 100 MPa (1000 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 104 nm3

SASDPA7 – Acidic leucine-rich nuclear phosphoprotein 32 family member A (pp32) at 4 °C and 5 MPa (1 atm)

Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) experimental SAS data
Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) Kratky plot
Sample: Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) monomer, 29 kDa Homo sapiens protein
Buffer: 25 mM BisTRIS, 10 mM NaCl, 5 mM DTT, pH: 6.8
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 1.8 nm
Dmax 6.0 nm
VolumePorod 31 nm3

SASDPB7 – Acidic leucine-rich nuclear phosphoprotein 32 family member A (pp32) at 4 °C and 100 MPa (1000 atm)

Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) experimental SAS data
Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) Kratky plot
Sample: Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) monomer, 29 kDa Homo sapiens protein
Buffer: 25 mM BisTRIS, 10 mM NaCl, 5 mM DTT, pH: 6.8
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.0 nm
Dmax 7.5 nm
VolumePorod 37 nm3

SASDPM4 – Apo SH2-SH3-SH2 domains from p120RasGAP

Ras GTPase-activating protein 1 experimental SAS data
DAMMIF model
Sample: Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8 350 mM NaCl 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 12
Tandem engagement of phosphotyrosines by the dual SH2 domains of p120RasGAP. Structure (2022)
Stiegler AL, Vish KJ, Boggon TJ
RgGuinier 2.6 nm
Dmax 10.3 nm
VolumePorod 41 nm3

SASDPN4 – Doubly Phosphorylated p190RhoGAP Peptide Bound to p120RasGAP's SH2-SH3-SH2 Domains

Ras GTPase-activating protein 1Rho GTPase-activating protein 35 experimental SAS data
DAMMIF model
Sample: Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
Rho GTPase-activating protein 35 monomer, 3 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8 350 mM NaCl 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 11
Tandem engagement of phosphotyrosines by the dual SH2 domains of p120RasGAP. Structure (2022)
Stiegler AL, Vish KJ, Boggon TJ
RgGuinier 2.4 nm
Dmax 7.9 nm
VolumePorod 41 nm3

SASDQL7 – Bicelles formed by DMPC DHPC

DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholineDMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Kratky plot
Sample: DHPC - 1,2-dihexanoyl-sn-glycero-3-phosphocholine None, lipid
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Jun 1
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M

SASDQM7 – Bicelles formed by DMPC cholate

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid experimental SAS data
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid Kratky plot
Sample: DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M

SASDQN7 – Bicelles formed by DMPC CHAPS

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate experimental SAS data
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate Kratky plot
Sample: DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M

SASDQP7 – Bicelles formed by DMPC DMPG and CHAPS

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonateDMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol experimental SAS data
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol Kratky plot
Sample: DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
CHAPS - 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate None, lipid
DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M

SASDQQ7 – Bicelles formed by DMPC DMPG and cholate

DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholineCholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acidDMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol experimental SAS data
DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol Kratky plot
Sample: DMPC - 1,2-dimyristoyl-sn-glycero-3-phosphocholine None, lipid
Cholate - 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid None, lipid
DMPG - 1,2-dimyristoyl-sn-glycero-3-phospho-sn-glycerol None, lipid
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 17
Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures Molecules 27(21):7628 (2022)
Giudice R, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M