Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Hybrid

SASDHX8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 6.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM histidine, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7

Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P

R_g^Guinier	4.6	nm
D_max	16.0	nm
Volume^Porod	258	nm³

SASDHY8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 7.5

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM histidine, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 26

R_g^Guinier	5.0	nm
D_max	17.4	nm
Volume^Porod	270	nm³

SASDHZ8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM TRIS, pH 8.5

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM TRIS, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Dec 15

R_g^Guinier	4.9	nm
D_max	16.7	nm
Volume^Porod	239	nm³

SASDH29 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM phosphate, pH 6.5

Sample:	Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer:	10 mM phosphate, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7

R_g^Guinier	4.9	nm
D_max	16.7	nm
Volume^Porod	240	nm³

SASDHG4 – NopAA, a type three effector from Sinorhizobium fredii USDA257 whith xyloglucanase activity

Type III effector NopAA experimental SAS data

Sample:	Type III effector NopAA monomer, 31 kDa Sinorhizobium fredii USDA257 protein
Buffer:	PBS, 150 mM NaCl, 10% glycerol, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2014 Dec 17

Structural and enzymatic characterisation of the Type III effector NopAA (=GunA) from Sinorhizobium fredii USDA257 reveals a Xyloglucan hydrolase activity. Sci Rep 10(1):9932 (2020)
Dorival J, Philys S, Giuntini E, Brailly R, de Ruyck J, Czjzek M, Biondi E, Bompard C

R_g^Guinier	2.4	nm
D_max	9.9	nm
Volume^Porod	38	nm³

SASDFP5 – DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 7.5 with 50 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha 16-mer, 153 kDa Escherichia coli protein
Buffer:	10 mM Bis-Tris, 50 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 May 27

Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

R_g^Guinier	8.9	nm
D_max	28.5	nm
Volume^Porod	410	nm³

SASDFQ5 – DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 7.5 with 100 mM NaCl

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha 16-mer, 153 kDa Escherichia coli protein
Buffer:	10 mM Bis-Tris, 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Jun 1

R_g^Guinier	6.6	nm
D_max	25.0	nm
Volume^Porod	336	nm³

SASDFR5 – DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 7.5 with 150 mM NaCl

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha 14-mer, 133 kDa Escherichia coli protein
Buffer:	10 mM Bis-Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Jun 1

R_g^Guinier	5.8	nm
D_max	24.2	nm
Volume^Porod	308	nm³

SASDFS5 – DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 7.5 with 300 mM NaCl

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha decamer, 95 kDa Escherichia coli protein
Buffer:	10 mM Bis-Tris, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Jun 1

R_g^Guinier	6.5	nm
D_max	24.0	nm
Volume^Porod	242	nm³

SASDFU5 – DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 6.5 with 100 mM NaCl

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha 14-mer, 133 kDa Escherichia coli protein
Buffer:	10 mM Bis-Tris, 100 mM NaCl, pH: 6.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Jun 1

R_g^Guinier	6.2	nm
D_max	24.4	nm
Volume^Porod	274	nm³

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