Browse by MODEL: Hybrid

SASDHH9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27
Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 76 nm3

SASDHJ9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and trehalose

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM trehalose, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27
Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.3 nm
VolumePorod 76 nm3

SASDHK9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and UDP-glucose

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM UDP-glucose, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27
Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
Mestrom L, Marsden S, van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 73 nm3

SASDJJ4 – Collagen VI von Willebrand factor (VWA) N2 domain

Collagen alpha-3(VI) chain, N2 domain experimental SAS data
DAMMIN model
Sample: Collagen alpha-3(VI) chain, N2 domain monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM TRIS, pH 7.4, 150mM NaCl 3% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 4
Structure of a collagen VI α3 chain VWA domain array: adaptability and functional implications of myopathy causing mutations Journal of Biological Chemistry :jbc.RA120.014865 (2020)
Solomon-Degefa H, Gebauer J, Jeffries C, Freiburg C, Meckelburg P, Bird L, Baumann U, Svergun D, Owens R, Werner J, Behrmann E, Paulsson M, Wagener R
RgGuinier 1.8 nm
Dmax 5.8 nm
VolumePorod 40 nm3

SASDLM9 – Minimal hepatocyte growth factor mimic K1K1

minimal hepatocyte growth factor mimic K1K1 experimental SAS data
GASBOR model
Sample: Minimal hepatocyte growth factor mimic K1K1 monomer, 19 kDa synthetic construct protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 3
Dimerization of kringle 1 domain from hepatocyte growth factor/scatter factor provides a potent MET receptor agonist Life Science Alliance 5(12):e202201424 (2022)
de Nola G, Leclercq B, Mougel A, Taront S, Simonneau C, Forneris F, Adriaenssens E, Drobecq H, Iamele L, Dubuquoy L, Melnyk O, Gherardi E, de Jonge H, Vicogne J
RgGuinier 2.2 nm
Dmax 6.6 nm
VolumePorod 22 nm3

SASDLN9 – Hepatocyte growth factor HGF/SF receptor - fragment MET567

Hepatocyte growth factor receptor experimental SAS data
GASBOR model
Sample: Hepatocyte growth factor receptor monomer, 62 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 27
Dimerization of kringle 1 domain from hepatocyte growth factor/scatter factor provides a potent MET receptor agonist Life Science Alliance 5(12):e202201424 (2022)
de Nola G, Leclercq B, Mougel A, Taront S, Simonneau C, Forneris F, Adriaenssens E, Drobecq H, Iamele L, Dubuquoy L, Melnyk O, Gherardi E, de Jonge H, Vicogne J
RgGuinier 3.2 nm
Dmax 11.7 nm
VolumePorod 126 nm3

SASDLP9 – Hepatocyte growth factor HGF/SF receptor - fragment MET567 in complex with the minimal hepatocyte growth factor mimic K1K1

minimal hepatocyte growth factor mimic K1K1Hepatocyte growth factor receptor experimental SAS data
CORAL model
Sample: Minimal hepatocyte growth factor mimic K1K1 monomer, 19 kDa synthetic construct protein
Hepatocyte growth factor receptor monomer, 62 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 23
Dimerization of kringle 1 domain from hepatocyte growth factor/scatter factor provides a potent MET receptor agonist Life Science Alliance 5(12):e202201424 (2022)
de Nola G, Leclercq B, Mougel A, Taront S, Simonneau C, Forneris F, Adriaenssens E, Drobecq H, Iamele L, Dubuquoy L, Melnyk O, Gherardi E, de Jonge H, Vicogne J
RgGuinier 3.7 nm
Dmax 14.3 nm
VolumePorod 136 nm3

SASDHR4 – A structural characterization of poly(aspartic acid) hydrolase-1 from Sphingomonas sp. KT-1

Poly(Aspartic acid) hydrolase-1 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Poly(Aspartic acid) hydrolase-1 dimer, 65 kDa Sphingomonas sp. KT-1 protein
Buffer: 20 mM Tris pH 7.0, 100 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 27
Structural Characterization of Sphingomonas sp. KT-1 PahZ1-Catalyzed Biodegradation of Thermally Synthesized Poly(aspartic acid) ACS Sustainable Chemistry & Engineering (2020)
Brambley C, Bolay A, Salvo H, Jansch A, Yared T, Miller J, Wallen J, Weiland M
RgGuinier 2.7 nm
Dmax 8.9 nm
VolumePorod 66 nm3

SASDHV8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.0

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 267 nm3

SASDHW8 – Human serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
Human serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 5.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 274 nm3