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SASDRY5 – Arabidopsis thaliana Toc75 Potra domains bound to synthetic antigen binding fragment sAB tc2

Protein TOC75-3, chloroplasticsynthetic antigen binding fragment tc2 experimental SAS data
Protein TOC75-3, chloroplastic synthetic antigen binding fragment tc2 Kratky plot
Sample: Protein TOC75-3, chloroplastic monomer, 35 kDa Arabidopsis thaliana protein
Synthetic antigen binding fragment tc2 monomer, 48 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 125 nm3

SASDRZ5 – Arabidopsis thaliana Toc75 Potra domains bound to synthetic antigen binding fragment sAB tc3

Protein TOC75-3, chloroplasticsynthetic antigen binding fragment tc3 experimental SAS data
Protein TOC75-3, chloroplastic synthetic antigen binding fragment tc3 Kratky plot
Sample: Protein TOC75-3, chloroplastic monomer, 35 kDa Arabidopsis thaliana protein
Synthetic antigen binding fragment tc3 monomer, 49 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.6 nm
Dmax 13.5 nm
VolumePorod 126 nm3

SASDR26 – Arabidopsis thaliana Toc75 Potra domains bound to synthetic antigen binding fragment sAB tc5

Protein TOC75-3, chloroplasticsynthetic antigen binding fragment tc5 experimental SAS data
Protein TOC75-3, chloroplastic synthetic antigen binding fragment tc5 Kratky plot
Sample: Protein TOC75-3, chloroplastic monomer, 35 kDa Arabidopsis thaliana protein
Synthetic antigen binding fragment tc5 monomer, 49 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.8 nm
Dmax 13.5 nm
VolumePorod 130 nm3

SASDR36 – Pisum sativum Toc75 Potra domains

Protein TOC75, chloroplastic experimental SAS data
Protein TOC75, chloroplastic Kratky plot
Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 2.9 nm
Dmax 11.0 nm
VolumePorod 60 nm3

SASDR46 – Pisum sativum Toc75 Potra domains bound to synthetic antigen binding fragment sAB ax9

Protein TOC75, chloroplasticsynthetic antigen binding fragment ax9 experimental SAS data
Protein TOC75, chloroplastic synthetic antigen binding fragment ax9 Kratky plot
Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein
Synthetic antigen binding fragment ax9 monomer, 49 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.8 nm
Dmax 13.5 nm
VolumePorod 123 nm3

SASDR56 – Pisum sativum Toc75 Potra domains bound to synthetic antigen binding fragment sAB ax14

Protein TOC75, chloroplasticsynthetic antigen binding fragment ax14 experimental SAS data
Protein TOC75, chloroplastic synthetic antigen binding fragment ax14 Kratky plot
Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein
Synthetic antigen binding fragment ax14 monomer, 48 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 4.1 nm
Dmax 15.5 nm
VolumePorod 150 nm3

SASDR66 – Pisum sativum Toc75 Potra domains bound to synthetic antigen binding fragment sAB ax15

Protein TOC75, chloroplasticsynthetic antigen binding fragment ax15 experimental SAS data
Protein TOC75, chloroplastic synthetic antigen binding fragment ax15 Kratky plot
Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein
Synthetic antigen binding fragment ax15 monomer, 48 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 123 nm3

SASDR76 – Pisum sativum Toc75 Potra domains bound to synthetic antigen binding fragment sAB ax17

Protein TOC75, chloroplasticsynthetic antigen binding fragment ax17 experimental SAS data
Protein TOC75, chloroplastic synthetic antigen binding fragment ax17 Kratky plot
Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein
Synthetic antigen binding fragment ax17 monomer, 49 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 118 nm3

SASDPJ3 – IMP-1 saRNA encoding NSP1-4 of VEEV and the spike glycoprotein of SARS-CoV-2

IMP-1 saRNA experimental SAS data
IMP-1 saRNA Kratky plot
Sample: IMP-1 saRNA monomer, 3720 kDa RNA
Buffer: 5 mM Sodium Citrate, pH: 6.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Apr 27
Biophysical characterisation of the structure of a SARS-CoV-2 self-amplifying—RNA (saRNA) vaccine Biology Methods and Protocols (2023)
Myatt D, Wharram L, Graham C, Liddell J, Branton H, Pizzey C, Cowieson N, Rambo R, Shattock R
RgGuinier 23.8 nm
Dmax 85.9 nm
VolumePorod 6265 nm3

SASDKD6 – NURS complex subunit red1

NURS complex subunit red1 experimental SAS data
NURS complex subunit red1 Kratky plot
Sample: NURS complex subunit red1 monomer, 8 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 20 mM HEPES, pH 7.5, 150 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 12
Mechanistic insights into RNA surveillance by the canonical poly(A) polymerase Pla1 of the MTREC complex. Nat Commun 14(1):772 (2023)
Soni K, Sivadas A, Horvath A, Dobrev N, Hayashi R, Kiss L, Simon B, Wild K, Sinning I, Fischer T
RgGuinier 2.5 nm
Dmax 8.7 nm
VolumePorod 12 nm3