Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: No model

SASDKZ5 – RNA Long Tetraloop Hairpin Triplex in 200 mM KCl

RNA Long Tetraloop Hairpin Triplex experimental SAS data

RNA Long Tetraloop Hairpin Triplex Kratky plot

Sample:	RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer:	200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20

RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L

R_g^Guinier	2.8	nm
D_max	10.3	nm

SASDK26 – RNA Long Tetraloop Hairpin Triplex in 5 mM MgCl2

Sample:	RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer:	5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20

RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L

R_g^Guinier	2.9	nm
D_max	18.2	nm

SASDJV3 – GTPase-Activation Protein (GAP) domain (73-204) of Vibrio cholerae Outer Protein E (VopE)

Outer membrane virulence protein yopE experimental SAS data

Outer membrane virulence protein yopE Kratky plot

Sample:	Outer membrane virulence protein yopE monomer, 15 kDa Vibrio cholerae protein
Buffer:	10 mM HEPES-HCl, pH 7.4, 150 mM NaCl, 0.5 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Jun 29

Solution structure and dynamics of the mitochondrial‐targeted GTPase ‐activating protein ( GAP ) VopE by an integrated NMR / SAXS approach Protein Science (2022)
Smith K, Lee W, Tonelli M, Lee Y, Light S, Cornilescu G, Chakravarthy S

R_g^Guinier	2.0	nm
D_max	8.0	nm
Volume^Porod	28	nm³

SASDM42 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles without Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data

Cobalt/magnesium transport protein CorA Kratky plot

Sample:	Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer:	20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23

Mg²⁺-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
Johansen NT, Bonaccorsi M, Bengtsen T, Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Lindorff-Larsen K, Pintacuda G, Arleth L

R_g^Guinier	4.2	nm
D_max	13.9	nm

SASDM52 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles with bound Mg2+

Sample:	Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer:	20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23

R_g^Guinier	4.3	nm
D_max	13.9	nm

SASDM62 – Cobalt/magnesium transport protein CorA in matched-out deuterated nanodiscs without Mg2+

Sample:	Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer:	20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23

R_g^Guinier	4.8	nm
D_max	14.2	nm

SASDM72 – Cobalt/magnesium transport protein CorA in match-out deuterated nanodiscs with bound Mg2+

Sample:	Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer:	20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23

R_g^Guinier	4.9	nm
D_max	14.3	nm

SASDMH4 – Arrestin-3 ERK2 fusion, dimer (Arrestin-3 fused to extracellular signal-regulated kinase 2)

Arrestin-3 fused to extracellular signal-regulated kinase 2 experimental SAS data

Arrestin-3 fused to extracellular signal-regulated kinase 2 Kratky plot

Sample:	Arrestin-3 fused to extracellular signal-regulated kinase 2 dimer, 178 kDa Bos taurus/Rattus norvegicus protein
Buffer:	20 mM MOPS pH 7.5, 150 mM NaCl, 1 mM TCEP, and 5% glycerol, pH: 7.5
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Mar 23

The two non-visual arrestins engage ERK2 differently. J Mol Biol :167465 (2022)
Perry-Hauser NA, Bennett Hopkins J, Zhuo Y, Zheng C, Perez I, Schultz KM, Vishnivetskiy SA, Kaya AI, Sharma P, Dalby KN, Young Chung K, Klug CS, Gurevich VV, Iverson TM

R_g^Guinier	4.3	nm
D_max	16.0	nm

SASDMY7 – Poly-histidine tagged Myosin X component at 8979 eV

Unconventional myosin-X component experimental SAS data

Unconventional myosin-X component Kratky plot

Sample:	Unconventional myosin-X component dimer, 15 kDa Homo sapiens protein
Buffer:	HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16

K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
Virk K, Yonezawa K, Choukate K, Singh L, Shimizu N, Chaudhuri B

R_g^Guinier	2.2	nm
D_max	8.5	nm
Volume^Porod	24	nm³

SASDMZ7 – Poly-histidine tagged Myosin X component at 8981 eV

Unconventional myosin-X experimental SAS data

Sample:	Unconventional myosin-X dimer, 15 kDa Homo sapiens protein
Buffer:	HEPES, 5% glycerol, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BL-15A2, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 Mar 16

K -edge anomalous SAXS for protein solution structure modeling Acta Crystallographica Section D Structural Biology 78(2) (2022)
Virk K, Yonezawa K, Choukate K, Singh L, Shimizu N, Chaudhuri B

R_g^Guinier	2.2	nm
D_max	8.0	nm
Volume^Porod	24	nm³

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