Browse by MODEL: No model

SASDK83 – double Element for Nuclear Expression (dENE) Mutant C + Poly(A)30

C dENE + Poly(A)30 experimental SAS data
C dENE + Poly(A)30 Kratky plot
Sample: C dENE + Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.6 nm
Dmax 14.8 nm

SASDKA3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)25

Xtal dENE + Poly(A)25 experimental SAS data
Xtal dENE + Poly(A)25 Kratky plot
Sample: Xtal dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKB3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)30

Xtal dENE + Poly(A)30 experimental SAS data
Xtal dENE + Poly(A)30 Kratky plot
Sample: Xtal dENE + Poly(A)30 monomer, 38 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 13.2 nm

SASDKC3 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)25

A dENE + Poly(A)25 experimental SAS data
A dENE + Poly(A)25 Kratky plot
Sample: A dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKD3 – double Element for Nuclear Expression (dENE) Mutant B + Hairpin - Poly(A)30

B dENE + Hairpin - Poly(A)30 experimental SAS data
B dENE + Hairpin - Poly(A)30 Kratky plot
Sample: B dENE + Hairpin - Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 14.0 nm

SASDKE3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)20

Xtal dENE + Poly(A)20 experimental SAS data
Xtal dENE + Poly(A)20 Kratky plot
Sample: Xtal dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.8 nm

SASDKT8 – von Willebrand Factor peptide 3mg/ml

von Willebrand factor experimental SAS data
von Willebrand factor Kratky plot
Sample: Von Willebrand factor monomer, 11 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 24
An Integrative Structural Biology Analysis of Von Willebrand Factor Binding and Processing by ADAMTS-13 in Solution. J Mol Biol 433(13):166954 (2021)
Del Amo-Maestro L, Sagar A, Pompach P, Goulas T, Scavenius C, Ferrero DS, Castrillo-Briceño M, Taulés M, Enghild JJ, Bernadó P, Gomis-Rüth FX
RgGuinier 3.1 nm
Dmax 14.0 nm
VolumePorod 28 nm3

SASDKZ4 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 8.4 nm
Dmax 29.0 nm

SASDK25 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe, 0.165 g/L

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 8.3 nm
Dmax 32.0 nm

SASDK35 – the Gemin2/Survival Motor Neuron-like protein 1 (SMN1) complex from Schizosaccharomyces pombe, 0.419 g/L

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: Survival motor neuron-like protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 8.2 nm
Dmax 29.7 nm