Browse by MODEL: No model

SASDF22 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.

somapacitanFcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagneonatal Fc receptor ectodomain alpha-chain experimental SAS data
somapacitan FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: Somapacitan monomer, 22 kDa Homo sapiens protein
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
Neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
Neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 May 11
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 4.2 nm
Dmax 14.7 nm
VolumePorod 227 nm3

SASDF32 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the albumin-binding side chain of the human growth hormone derivative somapacitan.

FcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagAlbumin-binding side-chainneonatal Fc receptor ectodomain alpha-chain experimental SAS data
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag Albumin-binding side-chain neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
Neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
Albumin-binding side-chain monomer, 1 kDa
Neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Nov 11
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 3.6 nm
Dmax 12.6 nm
VolumePorod 174 nm3

SASDGV2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D' and 2H Fibrillarin (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at KWS1, FRM2 on 2015 May 24
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 87 nm3

SASDGW2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D' and 2H Nop5 (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2015 Sep 21
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 210 nm3

SASDGX2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H Nop5, 1H st-sR26 RNA, 1H substrate D' and 2H L7Ae (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2015 Nov 23
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.0 nm
Dmax 13.3 nm
VolumePorod 42 nm3

SASDGY2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H Nop5, 1H L7Ae, 1H substrate D' and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jul 21
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 52 nm3

SASDGZ2 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H substrate D', 2H Fibrillarin and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at KWS1, FRM2 on 2015 May 23
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.8 nm
Dmax 16.6 nm
VolumePorod 82 nm3

SASDG23 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H L7Ae, 1H substrate D', 2H(70%) Nop5 and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D' experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D' Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D' monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Apr 8
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.0 nm
Dmax 13.8 nm
VolumePorod 214 nm3

SASDG33 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Fibrillarin, 1H L7Ae, 1H substrate D, 2H(70%) Nop5 and 2H st-sR26 RNA (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Apr 8
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.0 nm
Dmax 13.9 nm
VolumePorod 231 nm3

SASDG43 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D and 2H Fibrillarin (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase50S ribosomal protein L7AeNOP5/NOP56 related proteinpyrococcus furiosus sR26 stabilized constructPyrococcus furiosus sR26 substrate D experimental SAS data
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot
Sample: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase dimer, 52 kDa Pyrococcus furiosus protein
50S ribosomal protein L7Ae dimer, 27 kDa Pyrococcus furiosus protein
NOP5/NOP56 related protein dimer, 94 kDa Pyrococcus furiosus protein
Pyrococcus furiosus sR26 stabilized construct monomer, 24 kDa Pyrococcus furiosus RNA
Pyrococcus furiosus sR26 substrate D monomer, 4 kDa Pyrococcus furiosus RNA
Buffer: 50 mM phosphate 500 mM NaCl 42%D2O, pH: 6.6
Experiment: SANS data collected at KWS1, FRM2 on 2015 May 24
The guide sRNA sequence determines the activity level of box C/D RNPs. Elife 9 (2020)
Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T
RgGuinier 4.9 nm
Dmax 16.6 nm
VolumePorod 65 nm3