Browse by MODEL: No model

SASDN27 – ASO1 DNA in the presence target RNA

LNA-DNA-LNAPSCK9, 24mer ASO binding site experimental SAS data
LNA-DNA-LNA PSCK9, 24mer ASO binding site Kratky plot
Sample: LNA-DNA-LNA monomer, 4 kDa DNA
PSCK9, 24mer ASO binding site monomer, 8 kDa RNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 15 nm3

SASDN47 – ASO3 RNA in the presence of target RNA

oME ASOPSCK9, 24mer ASO binding site experimental SAS data
oME ASO PSCK9, 24mer ASO binding site Kratky plot
Sample: OME ASO monomer, 4 kDa RNA
PSCK9, 24mer ASO binding site monomer, 8 kDa RNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 16 nm3

SASDVV5 – Arabidopsis thaliana Auxin Response Factor 1 in complex with high affinity DNA

Auxin response factor 1High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing experimental SAS data
Auxin response factor 1 High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing Kratky plot
Sample: Auxin response factor 1 dimer, 81 kDa Arabidopsis thaliana protein
High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing dimer, 13 kDa DNA
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3
The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1. Structure (2025)
Crespo I, Malfois M, Rienstra J, Tarrés-Solé A, van den Berg W, Weijers D, Boer DR
RgGuinier 3.6 nm
Dmax 10.5 nm
VolumePorod 136 nm3

SASDWN8 – β-Lactoglobulin A at 17.4 µM (pH 7.0)

Beta-lactoglobulin experimental SAS data
Beta-lactoglobulin Kratky plot
Sample: Beta-lactoglobulin, 20 kDa Bos taurus protein
Buffer: 20 mM MOPS, 100 mM NaCl, 1 mM TCEP, pH:
Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 21
KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models Journal of Molecular Biology :169103 (2025)
Gomes T, Ruiz L, Martin-Malpartida P, Bernadó P, Baptista A, Macias M, Cordeiro T
RgGuinier 1.9 nm
Dmax 6.0 nm
VolumePorod 31 nm3

SASDWP8 – β-Lactoglobulin A at 26.1 µM (pH 7.0)

Beta-lactoglobulin experimental SAS data
Beta-lactoglobulin Kratky plot
Sample: Beta-lactoglobulin, 20 kDa Bos taurus protein
Buffer: 20 mM MOPS, 100 mM NaCl, 1 mM TCEP, pH:
Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 21
KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models Journal of Molecular Biology :169103 (2025)
Gomes T, Ruiz L, Martin-Malpartida P, Bernadó P, Baptista A, Macias M, Cordeiro T
RgGuinier 2.0 nm
Dmax 8.5 nm
VolumePorod 30 nm3

SASDWQ8 – β-Lactoglobulin A at 34.8 µM (pH 7.0)

Beta-lactoglobulin experimental SAS data
Beta-lactoglobulin Kratky plot
Sample: Beta-lactoglobulin, 20 kDa Bos taurus protein
Buffer: 20 mM MOPS, 100 mM NaCl, 1 mM TCEP, pH:
Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 21
KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models Journal of Molecular Biology :169103 (2025)
Gomes T, Ruiz L, Martin-Malpartida P, Bernadó P, Baptista A, Macias M, Cordeiro T
RgGuinier 2.0 nm
Dmax 8.5 nm
VolumePorod 33 nm3

SASDWR8 – β-Lactoglobulin A at 52.2 µM (pH 7.0)

Beta-lactoglobulin experimental SAS data
Beta-lactoglobulin Kratky plot
Sample: Beta-lactoglobulin, 20 kDa Bos taurus protein
Buffer: 20 mM MOPS, 100 mM NaCl, 1 mM TCEP, pH:
Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 21
KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models Journal of Molecular Biology :169103 (2025)
Gomes T, Ruiz L, Martin-Malpartida P, Bernadó P, Baptista A, Macias M, Cordeiro T
RgGuinier 2.1 nm
Dmax 9.0 nm
VolumePorod 40 nm3

SASDWS8 – β-Lactoglobulin A at 69.6 µM (pH 7.0)

Beta-lactoglobulin experimental SAS data
Beta-lactoglobulin Kratky plot
Sample: Beta-lactoglobulin, 20 kDa Bos taurus protein
Buffer: 20 mM MOPS, 100 mM NaCl, 1 mM TCEP, pH:
Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 21
KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models Journal of Molecular Biology :169103 (2025)
Gomes T, Ruiz L, Martin-Malpartida P, Bernadó P, Baptista A, Macias M, Cordeiro T
RgGuinier 2.1 nm
Dmax 9.0 nm
VolumePorod 44 nm3

SASDWT8 – β-Lactoglobulin A at 78.3 µM (pH 7.0)

Beta-lactoglobulin experimental SAS data
Beta-lactoglobulin Kratky plot
Sample: Beta-lactoglobulin, 20 kDa Bos taurus protein
Buffer: 20 mM MOPS, 100 mM NaCl, 1 mM TCEP, pH:
Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 21
KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models Journal of Molecular Biology :169103 (2025)
Gomes T, Ruiz L, Martin-Malpartida P, Bernadó P, Baptista A, Macias M, Cordeiro T
RgGuinier 2.1 nm
Dmax 10.0 nm
VolumePorod 45 nm3

SASDWU8 – β-Lactoglobulin A at 104.3 µM (pH 7.0)

Beta-lactoglobulin experimental SAS data
Beta-lactoglobulin Kratky plot
Sample: Beta-lactoglobulin, 20 kDa Bos taurus protein
Buffer: 20 mM MOPS, 100 mM NaCl, 1 mM TCEP, pH:
Experiment: SAXS data collected at BM29, ESRF on 2021 Nov 21
KDSAXS: A tool for Analyzing Binding Equilibria with SAXS Data using Explicit Models Journal of Molecular Biology :169103 (2025)
Gomes T, Ruiz L, Martin-Malpartida P, Bernadó P, Baptista A, Macias M, Cordeiro T
RgGuinier 2.1 nm
Dmax 9.5 nm
VolumePorod 47 nm3