Browse by MODEL: No model

SASDWD3 – Cyanobacterial circadian clock protein KaiB-KaiC complex (hydrogenated-KaiB and hydrogenated-KaiC in 100% v/v D₂O ) measured by SEC-SANS

Circadian clock oscillator protein KaiBCircadian clock oscillator protein KaiC experimental SAS data
Circadian clock oscillator protein KaiB Circadian clock oscillator protein KaiC Kratky plot
Sample: Circadian clock oscillator protein KaiB hexamer, 71 kDa Synechococcus sp. (strain … protein
Circadian clock oscillator protein KaiC hexamer, 356 kDa Synechococcus elongatus (strain … protein
Buffer: 50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D₂O, pH: 7.8
Experiment: SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Jul 2
Size-exclusion chromatography–small-angle neutron scattering system optimized for an instrument with medium neutron flux Journal of Applied Crystallography 58(2) (2025)
Morishima K, Inoue R, Nakagawa T, Shimizu M, Sakamoto R, Oda T, Mayumi K, Sugiyama M
RgGuinier 4.9 nm
Dmax 14.4 nm

SASDWE3 – Cyanobacterial circadian clock protein KaiB-KaiC complex (71% deuterated-KaiB and hydrogenated-KaiC in 100% v/v D₂O ) measured by SEC-SANS

Circadian clock oscillator protein KaiBCircadian clock oscillator protein KaiC experimental SAS data
Circadian clock oscillator protein KaiB Circadian clock oscillator protein KaiC Kratky plot
Sample: Circadian clock oscillator protein KaiB hexamer, 71 kDa Synechococcus sp. (strain … protein
Circadian clock oscillator protein KaiC hexamer, 356 kDa Synechococcus elongatus (strain … protein
Buffer: 50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D₂O, pH: 7.8
Experiment: SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Jul 2
Size-exclusion chromatography–small-angle neutron scattering system optimized for an instrument with medium neutron flux Journal of Applied Crystallography 58(2) (2025)
Morishima K, Inoue R, Nakagawa T, Shimizu M, Sakamoto R, Oda T, Mayumi K, Sugiyama M
RgGuinier 4.3 nm
Dmax 11.7 nm

SASDWF3 – Circadian clock protein KaiC (hydrogenated-KaiC in 100% v/v D₂O ) measured by SEC-SANS

Circadian clock oscillator protein KaiC experimental SAS data
Circadian clock oscillator protein KaiC Kratky plot
Sample: Circadian clock oscillator protein KaiC hexamer, 356 kDa Synechococcus elongatus (strain … protein
Buffer: 50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D₂O, pH: 7.8
Experiment: SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Jul 2
Size-exclusion chromatography–small-angle neutron scattering system optimized for an instrument with medium neutron flux Journal of Applied Crystallography 58(2) (2025)
Morishima K, Inoue R, Nakagawa T, Shimizu M, Sakamoto R, Oda T, Mayumi K, Sugiyama M
RgGuinier 4.4 nm
Dmax 11.9 nm

SASDTK7 – RECQ like helicase 5 (1 mg/ml)

ATP-dependent DNA helicase Q5 experimental SAS data
ATP-dependent DNA helicase Q5 Kratky plot
Sample: ATP-dependent DNA helicase Q5, 109 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Mar 29
Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5
Tomas Klumpler
RgGuinier 6.5 nm
Dmax 19.5 nm
VolumePorod 310 nm3

SASDTL7 – RECQ like helicase 5 (4 mg/ml)

ATP-dependent DNA helicase Q5 experimental SAS data
ATP-dependent DNA helicase Q5 Kratky plot
Sample: ATP-dependent DNA helicase Q5, 109 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Mar 29
Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5
Tomas Klumpler
RgGuinier 6.4 nm
Dmax 20.2 nm
VolumePorod 288 nm3

SASDTM7 – RECQ like helicase 5 fragment (amino acids 625-825; 0.2 mg/ml)

ATP-dependent DNA helicase Q5 experimental SAS data
ATP-dependent DNA helicase Q5 Kratky plot
Sample: ATP-dependent DNA helicase Q5, 21 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Apr 19
Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5
Tomas Klumpler
RgGuinier 3.7 nm
Dmax 9.3 nm
VolumePorod 73 nm3

SASDTN7 – RECQ like helicase 5 fragment (amino acids 625-825; 0.4 mg/ml)

ATP-dependent DNA helicase Q5 experimental SAS data
ATP-dependent DNA helicase Q5 Kratky plot
Sample: ATP-dependent DNA helicase Q5, 109 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Apr 19
Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5
Tomas Klumpler
RgGuinier 3.9 nm
Dmax 10.1 nm
VolumePorod 91 nm3

SASDTP7 – RECQ like helicase 5 fragment (amino acids 625-825; 0.6 mg/ml)

ATP-dependent DNA helicase Q5 experimental SAS data
ATP-dependent DNA helicase Q5 Kratky plot
Sample: ATP-dependent DNA helicase Q5, 109 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Apr 19
Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5
Tomas Klumpler
RgGuinier 4.3 nm
Dmax 11.2 nm
VolumePorod 116 nm3

SASDTQ7 – RECQ like helicase 5 fragment (amino acids 625-825; 0.8 mg/ml)

ATP-dependent DNA helicase Q5 experimental SAS data
ATP-dependent DNA helicase Q5 Kratky plot
Sample: ATP-dependent DNA helicase Q5, 109 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Apr 19
Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5
Tomas Klumpler
RgGuinier 4.6 nm
Dmax 13.2 nm
VolumePorod 102 nm3

SASDTR7 – RECQ like helicase 5 fragment (amino acids 625-825; 1.0 mg/ml)

ATP-dependent DNA helicase Q5 experimental SAS data
ATP-dependent DNA helicase Q5 Kratky plot
Sample: ATP-dependent DNA helicase Q5, 109 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Apr 19
Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5
Tomas Klumpler
RgGuinier 5.7 nm
Dmax 16.2 nm
VolumePorod 150 nm3