Browse by MODEL: No model

SASDTH7 – RNase P RNA in the presence of 8.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 8.0 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.7 nm
Dmax 14.8 nm
VolumePorod 280 nm3

SASDTJ7 – RNase P RNA in the presence of 10.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl,10.0 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.8 nm
Dmax 14.6 nm
VolumePorod 285 nm3

SASDQK7 – Alpha Synuclein at 283 K and concentration of 3.8 mg/ml

Human alpha-synuclein experimental SAS data
Human alpha-synuclein Kratky plot
Sample: Human alpha-synuclein monomer, 14 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 50 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2015 Mar 18
Visualizing gaussian-chain like structural models of human α-synuclein in monomeric pre-fibrillar state: Solution SAXS data and modeling analysis. Int J Biol Macromol 288:138614 (2024)
Dey M, Gupta A, Badmalia MD, Ashish, Sharma D
RgGuinier 4.7 nm
Dmax 18.0 nm
VolumePorod 76 nm3

SASDQV8 – Na/Ca-exchange protein - CALX1.2 CBD12 domain in the Ca2+-free (Apo) state

Na/Ca-exchange protein, isoform D experimental SAS data
Na/Ca-exchange protein, isoform D Kratky plot
Sample: Na/Ca-exchange protein, isoform D monomer, 31 kDa Drosophila melanogaster protein
Buffer: 20 mM TRIS, 200 mM NaCl, 1% v/v glycerol, 0.03% w/v NaN3, 1 mM β-mercaptoethanol, 2 mM EDTA, pH: 7.5
Experiment: SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Mar 8
SAXS characterisation of CALX1.2 CBD12 construct
Roberto Kopke Salinas
RgGuinier 2.6 nm
Dmax 10.0 nm
VolumePorod 48 nm3

SASDQW8 – Na/Ca-exchange protein - CALX1.2 CBD12 domain in the Ca2+-bound state

Na/Ca-exchange protein, isoform D experimental SAS data
Na/Ca-exchange protein, isoform D Kratky plot
Sample: Na/Ca-exchange protein, isoform D monomer, 31 kDa Drosophila melanogaster protein
Buffer: 20 mM TRIS, 200 mM NaCl, 1% v/v glycerol, 0.03% w/v NaN3, 1 mM β-mercaptoethanol, 50 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Mar 8
SAXS characterisation of CALX1.2 CBD12 construct
RgGuinier 2.7 nm
Dmax 9.9 nm
VolumePorod 32 nm3

SASDUL9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.6 nm
Dmax 11.6 nm
VolumePorod 23 nm3

SASDUM9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea

importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK experimental SAS data
importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK Kratky plot
Sample: Importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.6 nm
Dmax 13.8 nm
VolumePorod 18 nm3

SASDUN9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 6 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 29
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.7 nm
Dmax 11.9 nm
VolumePorod 23 nm3

SASDUP9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 2 M Urea

importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK experimental SAS data
importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK Kratky plot
Sample: Importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.0 nm
Dmax 14.4 nm
VolumePorod 31 nm3

SASDUQ9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 2 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.7 nm
Dmax 11.8 nm
VolumePorod 22 nm3