Browse by MODEL: No model

SASDRU4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) trimer formed in the presence of L-serine

Phosphoserine phosphatase SerB2 experimental SAS data
Phosphoserine phosphatase SerB2 Kratky plot
Sample: Phosphoserine phosphatase SerB2 trimer, 130 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, 10 mM L-serine, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 4.0 nm
Dmax 14.3 nm
VolumePorod 200 nm3

SASDRV4 – Mycobacterium marinum phosphoserine phosphatase (MmSerB2) dimer

phosphoserine phosphatase experimental SAS data
phosphoserine phosphatase Kratky plot
Sample: Phosphoserine phosphatase dimer, 88 kDa Mycobacterium marinum (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 3.2 nm
Dmax 9.9 nm
VolumePorod 138 nm3

SASDSR8 – Calcium/calmodulin-dependent protein kinase kinase 1 (CaMKK1)

Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) experimental SAS data
Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 3.2 nm
Dmax 10.7 nm
VolumePorod 114 nm3

SASDSS8 – Phosphorylated Calcium/calmodulin-dependent protein kinase 1 (pCaMKK1)

Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) experimental SAS data
Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 3.0 nm
Dmax 10.5 nm
VolumePorod 97 nm3

SASDST8 – Phosphorylated Calcium/calmodulin-dependent protein kinase 2 (pCaMKK2)

Calcium/calmodulin-dependent protein kinase kinase 2 experimental SAS data
Calcium/calmodulin-dependent protein kinase kinase 2 Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T
RgGuinier 3.0 nm
Dmax 12.0 nm
VolumePorod 87 nm3

SASDQZ9 – Beclin-1 amino acids 1-150 with two cysteine motifs, along with a C-terminal tyrosine analysed using EFA in BIOXTAS RAW software, component 0

Beclin-1 experimental SAS data
Beclin-1 Kratky plot
Sample: Beclin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM Tris, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 12
Invariant BECN1 CXXC motifs bind Zn(2+) and regulate structure and function of the BECN1 intrinsically disordered region. Autophagy :1-17 (2023)
Mukhopadhyay S, Subedi S, Hopkins JB, Ugrinov A, Chakravarthy S, Colbert CL, Sinha SC
RgGuinier 4.3 nm
Dmax 20.0 nm
VolumePorod 87 nm3

SASDRV7 – SARS-CoV-2 5' untranslated region stem-loop 4 RNA (5_SL4) in phosphate buffer

stem-loop 4 from SARS-CoV-2 5'-UTR experimental SAS data
stem-loop 4 from SARS-CoV-2 5'-UTR Kratky plot
Sample: Stem-loop 4 from SARS-CoV-2 5'-UTR monomer, 14 kDa SARS coronavirus 2-Wuhan RNA
Buffer: 25 mM potassium phosphate pH 6.5, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
High-resolution structure of stem-loop 4 from the 5'-UTR of SARS-CoV-2 solved by solution state NMR. Nucleic Acids Res (2023)
Vögele J, Hymon D, Martins J, Ferner J, Jonker HRA, Hargrove AE, Weigand JE, Wacker A, Schwalbe H, Wöhnert J, Duchardt-Ferner E
RgGuinier 2.1 nm
Dmax 7.0 nm
VolumePorod 22 nm3

SASDS97 – polymerase alpha-primase polΔcat-prim bound to DNA in the presence of RNA initiation factors

DNA polymerase alpha subunit BDNA polymerase alpha catalytic subunitDNA primase large subunitDNA primase small subunit22mer DNA template experimental SAS data
DNA polymerase alpha subunit B DNA polymerase alpha catalytic subunit DNA primase large subunit DNA primase small subunit 22mer DNA template Kratky plot
Sample: DNA polymerase alpha subunit B monomer, 49 kDa Homo sapiens protein
DNA polymerase alpha catalytic subunit monomer, 23 kDa Homo sapiens protein
DNA primase large subunit monomer, 59 kDa Homo sapiens protein
DNA primase small subunit monomer, 50 kDa Homo sapiens protein
22mer DNA template monomer, 7 kDa DNA
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MnCl2, 1 mM TCEP, 1 mM 5'-guanylylmethylenediphosphonate (GMPCPP), pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Dec 4
Flexibility and distributive synthesis regulate RNA priming and handoff in human DNA polymerase α-primase Journal of Molecular Biology :168330 (2023)
Cordoba J, Mullins E, Salay L, Eichman B, Chazin W
RgGuinier 4.6 nm
Dmax 14.2 nm
VolumePorod 310 nm3

SASDSA7 – polymerase alpha-primase polΔcat-prim bound to DNA in the presence of an RNA-primed template

DNA polymerase alpha subunit BDNA polymerase alpha catalytic subunitDNA primase large subunitDNA primase small subunit29 mer DNA template8mer RNA primer experimental SAS data
DNA polymerase alpha subunit B DNA polymerase alpha catalytic subunit DNA primase large subunit DNA primase small subunit 29 mer DNA template 8mer RNA primer Kratky plot
Sample: DNA polymerase alpha subunit B monomer, 49 kDa Homo sapiens protein
DNA polymerase alpha catalytic subunit monomer, 23 kDa Homo sapiens protein
DNA primase large subunit monomer, 59 kDa Homo sapiens protein
DNA primase small subunit monomer, 50 kDa Homo sapiens protein
29 mer DNA template monomer, 9 kDa DNA
8mer RNA primer monomer, 3 kDa RNA
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 28
Flexibility and distributive synthesis regulate RNA priming and handoff in human DNA polymerase α-primase Journal of Molecular Biology :168330 (2023)
Cordoba J, Mullins E, Salay L, Eichman B, Chazin W
RgGuinier 4.9 nm
Dmax 15.3 nm
VolumePorod 344 nm3

SASDSB7 – polymerase alpha-primase polΔcat-primΔ2C in the absence of substrate

DNA polymerase alpha subunit BDNA polymerase alpha catalytic subunitDNA primase small subunitDNA primase large subunit experimental SAS data
DNA polymerase alpha subunit B DNA polymerase alpha catalytic subunit DNA primase small subunit DNA primase large subunit Kratky plot
Sample: DNA polymerase alpha subunit B monomer, 49 kDa Homo sapiens protein
DNA polymerase alpha catalytic subunit monomer, 23 kDa Homo sapiens protein
DNA primase small subunit monomer, 50 kDa Homo sapiens protein
DNA primase large subunit monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Apr 15
Flexibility and distributive synthesis regulate RNA priming and handoff in human DNA polymerase α-primase Journal of Molecular Biology :168330 (2023)
Cordoba J, Mullins E, Salay L, Eichman B, Chazin W
RgGuinier 4.5 nm
Dmax 14.5 nm
VolumePorod 221 nm3