|
|
|
|
|
| Sample: |
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
|
| Buffer: |
25 mM HEPES, 1 mM TCEP, pH: 7.5 |
| Experiment: |
SAXS
data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
|
The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease.
Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
|
| RgGuinier |
2.4 |
nm |
| Dmax |
7.8 |
nm |
| VolumePorod |
63 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
|
| Buffer: |
25 mM HEPES, 1 mM TCEP, pH: 7.5 |
| Experiment: |
SAXS
data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
|
The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease.
Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
|
| RgGuinier |
2.5 |
nm |
| Dmax |
8.2 |
nm |
| VolumePorod |
74 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
|
| Buffer: |
25 mM HEPES, 1 mM TCEP, pH: 7.5 |
| Experiment: |
SAXS
data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
|
The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease.
Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
|
| RgGuinier |
2.6 |
nm |
| Dmax |
8.2 |
nm |
| VolumePorod |
85 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
|
| Buffer: |
25 mM HEPES, 1 mM TCEP, pH: 7.5 |
| Experiment: |
SAXS
data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
|
The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease.
Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
|
| RgGuinier |
2.6 |
nm |
| Dmax |
7.7 |
nm |
| VolumePorod |
98 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
|
| Buffer: |
10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5 |
| Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2022 Nov 25
|
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering
International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
|
| RgGuinier |
1.7 |
nm |
| Dmax |
5.9 |
nm |
| VolumePorod |
21 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) monomer, 14 kDa Severe acute respiratory … protein
|
| Buffer: |
10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5 |
| Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2022 Nov 25
|
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering
International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
|
| RgGuinier |
1.6 |
nm |
| Dmax |
5.4 |
nm |
| VolumePorod |
19 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) monomer, 13 kDa Severe acute respiratory … protein
|
| Buffer: |
10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5 |
| Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2022 Nov 25
|
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering
International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
|
| RgGuinier |
1.5 |
nm |
| Dmax |
5.1 |
nm |
| VolumePorod |
16 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Replicase polyprotein 1ab (Non-structural protein 10) monomer, 16 kDa Severe acute respiratory … protein
|
| Buffer: |
10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5 |
| Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2023 Feb 6
|
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering
International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
|
| RgGuinier |
1.7 |
nm |
| Dmax |
5.9 |
nm |
| VolumePorod |
19 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Middle East respiratory … protein
|
| Buffer: |
10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5 |
| Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2023 Feb 6
|
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering
International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
|
| RgGuinier |
1.7 |
nm |
| Dmax |
5.6 |
nm |
| VolumePorod |
19 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
|
| Buffer: |
20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, pH: 7 |
| Experiment: |
SAXS
data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
|
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
|
|
|
