Browse by MODEL: No model

SASDJH7 – SAM2 Riboswitch 2 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 2 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDJJ7 – SAM2 Riboswitch 3 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDJK7 – SAM2 Riboswitch 3.75 mM MgCl2 Saturating SAM

S-adenosylmethionine riboswitch II experimental SAS data
S-adenosylmethionine riboswitch II Kratky plot
Sample: S-adenosylmethionine riboswitch II monomer, 17 kDa RNA
Buffer: 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 3.75 mM MgCl2 SAM, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Dec 10
SAXS Measurements of the SAM2 Riboswitch
Alex Plumridge
RgGuinier 2.0 nm

SASDSA5 – dark-state short-linker LOV-activated diguanylate cyclase

Diguanylate cyclase experimental SAS data
Diguanylate cyclase Kratky plot
Sample: Diguanylate cyclase dimer, 67 kDa Aquella oligotrophica protein
Buffer: 10 mM Tris, 500 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 3.4 nm
Dmax 13.6 nm
VolumePorod 102 nm3

SASDSB5 – light-state short-linker LOV-activated diguanylate cyclase

Diguanylate cyclase experimental SAS data
Diguanylate cyclase Kratky plot
Sample: Diguanylate cyclase dimer, 67 kDa Aquella oligotrophica protein
Buffer: 10 mM Tris, 500 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 3.4 nm
Dmax 15.7 nm
VolumePorod 104 nm3

SASDSD5 – light-state long-linker LOV-activated diguanylate cyclase

Sensor domain-containing diguanylate cyclase experimental SAS data
Sensor domain-containing diguanylate cyclase Kratky plot
Sample: Sensor domain-containing diguanylate cyclase dimer, 72 kDa Methylotenera sp. protein
Buffer: 10 mM Tris, 50 mM NaCl, 2 mM MgCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 May 4
Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases Science Advances 9(31) (2023)
Vide U, Kasapović D, Fuchs M, Heimböck M, Totaro M, Zenzmaier E, Winkler A
RgGuinier 3.5 nm
Dmax 13.8 nm
VolumePorod 123 nm3

SASDPB5 – SAXS curve of RNA Xist A-repeats (Liu buffer)

Xist A-repeat lncRNA experimental SAS data
Xist A-repeat lncRNA Kratky plot
Sample: Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer: 25 mM K-HEPES, 0.1 mM Na-EDTA, 150 mM KCl, 15 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 12.1 nm
Dmax 45.0 nm

SASDPC5 – SAXS curve of RNA Xist A-repeats (Smola buffer)

Xist A-repeat lncRNA experimental SAS data
Xist A-repeat lncRNA Kratky plot
Sample: Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer: 100 mM HEPES, 100 mM NaCl, 10 mM MgCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 9.8 nm
Dmax 40.0 nm
VolumePorod 657 nm3

SASDPD5 – SAXS curve of RNA Xist A-repeats (Maenner buffer)

Xist A-repeat lncRNA experimental SAS data
Xist A-repeat lncRNA Kratky plot
Sample: Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer: 20 mM HEPES-KOH, 100 mM KCl, 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF, 20% glycerol, 3.25 mM MgCl2, pH: 7.9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 8.9 nm
Dmax 35.0 nm
VolumePorod 414 nm3

SASDPE5 – SAXS curve of RNA Xist A-repeats (Duszczyk buffer)

Xist A-repeat lncRNA experimental SAS data
Xist A-repeat lncRNA Kratky plot
Sample: Xist A-repeat lncRNA monomer, 148 kDa Homo sapiens RNA
Buffer: 10 mM NaH2PO4/Na2HPO4, 100 mM NaCl, 0.02 mM EDTA, 0.02% azide, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 20
Conformation and structural dynamics of the Xist lncRNA A-repeats (2023)
Jones A, Gabel F, Bohn S, Wolfe G, Sattler M
RgGuinier 7.6 nm
Dmax 30.0 nm
VolumePorod 370 nm3