Small Angle Scattering Biological Data Bank SASBDB

MACROMOLECULE TYPE: heterocomplex

SASDAC7 – Complex LytTR-comcde

comcdeResponse regulator experimental SAS data

Sample:	Comcde, 24 kDa Streptococcus pneumoniae DNA Response regulator dimer, 29 kDa Streptococcus pneumoniae protein
Buffer:	50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Jun 2

Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
Sanchez D, Boudes M, van Tilbeurgh H, Durand D, Quevillon-Cheruel S

R_g^Guinier	3.6	nm
D_max	12.5	nm
Volume^Porod	74	nm³

SASDLT5 – Modification methylase SsoII (M.SsoII) protein bound to 12-bp DNA

Modification methylase SsoII12-bp DNA experimental SAS data

Modification methylase SsoII (M.SsoII) protein bound to 12-bp DNA Rg histogram

Sample:	Modification methylase SsoII monomer, 43 kDa Shigella sonnei protein 12-bp DNA monomer, 8 kDa DNA
Buffer:	50 mM Na-phosphate buffer, pH: 7
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Mar 13

Flexibility of the linker between the domains of DNA methyltransferase SsoII revealed by small-angle X-ray scattering: implications for transcription regulation in SsoII restriction-modification system. PLoS One 9(4):e93453 (2014)
Konarev PV, Kachalova GS, Ryazanova AY, Kubareva EA, Karyagina AS, Bartunik HD, Svergun DI

R_g^Guinier	2.8	nm
D_max	11.0	nm
Volume^Porod	85	nm³

SASDMZ4 – Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 2.9 mg/ml

Estrogen-related receptor gammaInverse repeat IR3 DNA experimental SAS data

Sample:	Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein Inverse repeat IR3 DNA trimer, 46 kDa DNA
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Mar 3

Reconstruction of Quaternary Structure from X-ray Scattering by Equilibrium Mixtures of Biological Macromolecules Biochemistry 52(39):6844-6855 (2013)
Petoukhov M, Billas I, Takacs M, Graewert M, Moras D, Svergun D

R_g^Guinier	4.7	nm
D_max	14.6	nm

SASDM25 – Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 1.9 mg/ml

Sample:	Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein Inverse repeat IR3 DNA trimer, 46 kDa DNA
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Mar 3

R_g^Guinier	4.3	nm
D_max	13.2	nm

SASDM35 – Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 1.0 mg/ml

Sample:	Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein Inverse repeat IR3 DNA trimer, 46 kDa DNA
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Mar 3

R_g^Guinier	4.1	nm
D_max	12.8	nm

SASDAH5 – Complex of Hfq with DsrA

RNA chaperone HfqRNA DsrA experimental SAS data

Sample:	RNA chaperone Hfq hexamer, 67 kDa Escherichia coli protein RNA DsrA monomer, 12 kDa RNA
Buffer:	50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Nov 16

Structural flexibility of RNA as molecular basis for Hfq chaperone function. Nucleic Acids Res 40(16):8072-84 (2012)
Ribeiro Ede A Jr, Beich-Frandsen M, Konarev PV, Shang W, Vecerek B, Kontaxis G, Hämmerle H, Peterlik H, Svergun DI, Bläsi U, Djinović-Carugo K

R_g^Guinier	4.3	nm
D_max	14.5	nm
Volume^Porod	210	nm³

SASDLU5 – Complex formed between the replication origin-binding protein UL9 (C-terminal domains) with 15-bp DNA

Replication origin-binding protein15-bp DNA experimental SAS data

Sample:	Replication origin-binding protein dimer, 81 kDa Human herpesvirus 1 … protein 15-bp DNA monomer, 9 kDa DNA
Buffer:	20 mM Tris-HCl, 50 mM NaCl, 5 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 May 27

Structural and biophysical characterization of the proteins interacting with the herpes simplex virus 1 origin of replication. J Biol Chem 284(24):16343-16353 (2009)
Manolaridis I, Mumtsidu E, Konarev P, Makhov AM, Fullerton SW, Sinz A, Kalkhof S, McGeehan JE, Cary PD, Griffith JD, Svergun D, Kneale GG, Tucker PA

R_g^Guinier	3.6	nm
D_max	15.0	nm

SASDL27 – N-Oct-3 POU transcription factor domain in complex with rat CRH DNA

POU domain, class 3, transcription factor 2Rat CRH DNA experimental SAS data

Sample:	POU domain, class 3, transcription factor 2 monomer, 19 kDa Homo sapiens protein Rat CRH DNA monomer, 15 kDa Rattus norvegicus DNA
Buffer:	50 mM Tris, 0.4 M NaCl, 2% glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2003 Oct 9

Fine-tuning of intrinsic N-Oct-3 POU domain allostery by regulatory DNA targets Nucleic Acids Research 35(13):4420-4432 (2007)
Alazard R, Mourey L, Ebel C, Konarev P, Petoukhov M, Svergun D, Erard M

R_g^Guinier	2.9	nm
D_max	11.0	nm
Volume^Porod	41	nm³

SASDL37 – N-Oct-3 POU transcription factor domain in complex with human DR-alpha DNA

POU domain, class 3, transcription factor 2Human DR-alpha DNA experimental SAS data

Sample:	POU domain, class 3, transcription factor 2 monomer, 19 kDa Homo sapiens protein Human DR-alpha DNA monomer, 15 kDa Homo sapiens DNA
Buffer:	50 mM Tris, 0.4 M NaCl, 2% glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2004 May 26

R_g^Guinier	2.9	nm
D_max	11.0	nm
Volume^Porod	44	nm³

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