Small Angle Scattering Biological Data Bank SASBDB

MACROMOLECULE TYPE: heterocomplex

SASDWS5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV) bound to stimulatory element RNA

Genome polyproteinStimulatory element RNA experimental SAS data

Sample:	Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein Stimulatory element RNA monomer, 12 kDa synthetic RNA RNA
Buffer:	20 mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT, pH:
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26

A new protein-dependent riboswitch activates ribosomal frameshifting (2025)
Betts J, Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C

R_g^Guinier	2.2	nm
D_max	7.5	nm
Volume^Porod	46	nm³

SASDSX5 – Nucleosome core particle (NCP) composed of the Widom 601 DNA

Histone H3.2Histone H4Histone H2AHistone H2B 1.1Widom 601 DNA sequence experimental SAS data

Sample:	Histone H3.2 dimer, 31 kDa Xenopus laevis protein Histone H4 dimer, 23 kDa Xenopus laevis protein Histone H2A dimer, 28 kDa Xenopus laevis protein Histone H2B 1.1 dimer, 28 kDa Xenopus laevis protein Widom 601 DNA sequence dimer, 89 kDa Escherichia coli DNA
Buffer:	20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Dec 2

BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle
Asgar Abbas kazrani

R_g^Guinier	4.3	nm
D_max	12.3	nm
Volume^Porod	350	nm³

SASDSY5 – Nucleosome (NCP) in complex with B cell lymphoma 7 protein family member A (BCL7A)

Isoform 2 of B-cell CLL/lymphoma 7 protein family member AHistone H3.2Histone H4Histone H2AHistone H2B 1.1Widom 601 DNA sequence experimental SAS data

Sample:	Isoform 2 of B-cell CLL/lymphoma 7 protein family member A monomer, 25 kDa Homo sapiens protein Histone H3.2 dimer, 31 kDa Xenopus laevis protein Histone H4 dimer, 23 kDa Xenopus laevis protein Histone H2A dimer, 28 kDa Xenopus laevis protein Histone H2B 1.1 dimer, 28 kDa Xenopus laevis protein Widom 601 DNA sequence dimer, 89 kDa Escherichia coli DNA
Buffer:	20 mM Tris-HCl , 50 mM KCl, 1 mM DTT, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Dec 2

BCL7 proteins, novel subunits of the mammalian SWI/SNF complex, bind the nucleosome core particle
Asgar Abbas kazrani

R_g^Guinier	4.7	nm
D_max	24.4	nm
Volume^Porod	400	nm³

SASDS33 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 5 mg/mL

C3H1-type domain-containing proteinsingle stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data

C3H1-type domain-containing protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot

Sample:	C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9

Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks

R_g^Guinier	1.6	nm
D_max	5.6	nm
Volume^Porod	13	nm³

SASDS43 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 3 mg/mL

single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequenceC3H1-type domain-containing protein experimental SAS data

single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence C3H1-type domain-containing protein Kratky plot

Sample:	Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9

R_g^Guinier	1.5	nm
D_max	5.7	nm
Volume^Porod	13	nm³

SASDS53 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 2 mg/mL

Sample:	C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9

R_g^Guinier	1.5	nm
D_max	5.6	nm
Volume^Porod	13	nm³

SASDS63 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 6 mg/mL

Sample:	C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9

R_g^Guinier	1.5	nm
D_max	5.1	nm
Volume^Porod	13	nm³

SASDS73 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 4.5 mg/mL

Sample:	C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9

R_g^Guinier	1.5	nm
D_max	4.9	nm
Volume^Porod	13	nm³

SASDS83 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 3 mg/mL

Sample:	C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9

R_g^Guinier	1.5	nm
D_max	5.3	nm
Volume^Porod	13	nm³

SASDW97 – Recombination directionality factor (6H-RdfS) from M.japonicum bound to 40mer DNA

Recombination directionality factor RdfSattP_8 40-mer DNA experimental SAS data

Sample:	Recombination directionality factor RdfS tetramer, 52 kDa Mesorhizobium japonicum R7A protein AttP_8 40-mer DNA dimer, 25 kDa DNA
Buffer:	150 mM Tris-HCl, 300 mM NaCl, 5% v/v glycerol, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 19

Structural basis for control of integrative and conjugative element excision and transfer by the oligomeric winged helix–turn–helix protein RdfS Nucleic Acids Research 53(6) (2025)
Verdonk C, Agostino M, Eto K, Hall D, Bond C, Ramsay J

R_g^Guinier	3.8	nm
D_max	13.8	nm
Volume^Porod	134	nm³

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