Browse by MACROMOLECULE type: nucleic acid

SASDTE7 – RNase P RNA in the presence of 2.5 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 2.5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.9 nm
Dmax 15.5 nm
VolumePorod 306 nm3

SASDTF7 – RNase P RNA in the presence of 3.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 3 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.9 nm
Dmax 15.5 nm
VolumePorod 301 nm3

SASDTG7 – RNase P RNA in the presence of 5.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.7 nm
Dmax 14.6 nm
VolumePorod 277 nm3

SASDTH7 – RNase P RNA in the presence of 8.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 8.0 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.7 nm
Dmax 14.8 nm
VolumePorod 280 nm3

SASDTJ7 – RNase P RNA in the presence of 10.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl,10.0 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
Lee Y, Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.8 nm
Dmax 14.6 nm
VolumePorod 285 nm3

SASDVX2 – Full-length SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

full stem-loop 5 of SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: Full stem-loop 5 of SARS-CoV-2 5'genomic end monomer, 48 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
Mertinkus KR, Oxenfarth A, Richter C, Wacker A, Mata CP, Carazo JM, Schlundt A, Schwalbe H
RgGuinier 4.3 nm
Dmax 13.8 nm

SASDVY2 – Sub-element stem-loop 5a within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

sub-element stem-loop 5a from SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: Sub-element stem-loop 5a from SARS-CoV-2 5'genomic end monomer, 11 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
Mertinkus KR, Oxenfarth A, Richter C, Wacker A, Mata CP, Carazo JM, Schlundt A, Schwalbe H
RgGuinier 1.9 nm
Dmax 6.0 nm

SASDVC3 – Sub-element stem-loop 5b within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

sub-element stem-loop 5b from SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: Sub-element stem-loop 5b from SARS-CoV-2 5'genomic end monomer, 8 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 3
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
Mertinkus KR, Oxenfarth A, Richter C, Wacker A, Mata CP, Carazo JM, Schlundt A, Schwalbe H
RgGuinier 1.4 nm
Dmax 4.3 nm

SASDGE6 – G-quadruplex DNA from Hepatitis B virus (wild type)

G-quadrupex experimental SAS data
DAMFILT model
Sample: G-quadrupex monomer, 6 kDa Hepatitis B virus DNA
Buffer: 20 mM HEPES, 100 mM KCl, 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jan 15
G-quadruplex from HBV genome
Trushar Patel
RgGuinier 1.7 nm
Dmax 4.0 nm
VolumePorod 13 nm3

SASDGF6 – G-quadruplex DNA from Hepatitis B virus (mutant)

G-quadruplex mutant experimental SAS data
DAMFILT model
Sample: G-quadruplex mutant monomer, 6 kDa Hepatitis B virus DNA
Buffer: 20 mM HEPES, 100 mM KCl, 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jan 15
G-quadruplex from HBV genome
Trushar Patel
RgGuinier 1.7 nm
Dmax 5.5 nm
VolumePorod 11 nm3