Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: nucleic acid

SASDWC4 – 100 nm polystyrene beads from AF4-SAXS measurements

Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm experimental SAS data

Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm Kratky plot

Sample:	Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm monomer, 0 kDa synthetic construct
Buffer:	water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19

AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline. J Synchrotron Radiat (2025)
Da Vela S, Bartels K, Franke D, Soloviov D, Gräwert T, Molodenskiy D, Kolb B, Wilhelmy C, Drexel R, Meier F, Haas H, Langguth P, Graewert MA

R_g^Guinier	47.7	nm
D_max	111.0	nm

SASDWD4 – 20 nm polystyrene beads from AF4-SAXS measurements

Thermo Fisher polystyrene nanospheres with a diameter of 20 nm experimental SAS data

Thermo Fisher polystyrene nanospheres with a diameter of 20 nm Kratky plot

Sample:	Thermo Fisher polystyrene nanospheres with a diameter of 20 nm monomer, 1 kDa synthetic construct
Buffer:	water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19

R_g^Guinier	9.0	nm
D_max	25.0	nm

SASDWL5 – I-RNA, an A-to-I hyper-edited 20mer dsRNA

I-RNA, Top-strandI-RNA, Bottom-strand experimental SAS data

I-RNA, Top-strand I-RNA, Bottom-strand Kratky plot

Sample:	I-RNA, Top-strand monomer, 7 kDa RNA I-RNA, Bottom-strand monomer, 7 kDa RNA
Buffer:	25 mM sodium phosphate 25 mM sodium chloride, pH: 6.4
Experiment:	SAXS data collected at BM29, ESRF on 2022 Nov 9

Unique conformational dynamics and protein recognition of A-to-I hyper-edited dsRNA. Nucleic Acids Res 53(12) (2025)
Müller-Hermes C, Piomponi V, Hilber S, Asami S, Kreutz C, Bussi G, Sattler M

R_g^Guinier	2.0	nm
D_max	6.9	nm
Volume^Porod	17	nm³

SASDWM5 – A-RNA, non-edited 20mer dsRNA

A-RNA, Top-StrandA-RNA, Bottom-Strand experimental SAS data

A-RNA, Top-Strand A-RNA, Bottom-Strand Kratky plot

Sample:	A-RNA, Top-Strand monomer, 6 kDa RNA A-RNA, Bottom-Strand monomer, 6 kDa RNA
Buffer:	25 mM sodium phosphate 25 mM sodium chloride, pH: 6.4
Experiment:	SAXS data collected at BM29, ESRF on 2022 Nov 9

R_g^Guinier	1.8	nm
D_max	6.2	nm
Volume^Porod	17	nm³

SASDSY2 – single stranded 9-mer RNA: tristetraprolin TZF domain optimal target sequence, pH 8 at 17.1 mg/mL

single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data

single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot

Sample:	Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 19

Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks

R_g^Guinier	1.0	nm
D_max	3.5	nm
Volume^Porod	5	nm³

SASDSZ2 – single stranded 9-mer RNA: tristetraprolin TZF domain optimal target sequence, pH 8 at 17.1 mg/mL (repeat)

Sample:	Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 19

R_g^Guinier	1.0	nm
D_max	3.5	nm
Volume^Porod	5	nm³

SASDS23 – single stranded 9-mer RNA: tristetraprolin TZF domain optimal target sequence, pH 8 at 14 mg/mL

Sample:	Single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 19

R_g^Guinier	1.0	nm
D_max	3.2	nm
Volume^Porod	5	nm³

SASDU59 – BC120 RNA

Sample:	BC120 RNA monomer monomer, 39 kDa RNA
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Sep 22

Alu RNA pseudoknot alterations influence SRP9/SRP14 association. RNA (2025)
Gussakovsky D, Brown MJF, Pereira HS, Meier M, Padilla-Meier GP, Black NA, Booy EP, Stetefeld J, Patel TR, McKenna SA

R_g^Guinier	4.0	nm
D_max	14.3	nm
Volume^Porod	57	nm³

SASDU69 – EB120 RNA

Sample:	EB120 RNA monomer monomer, 39 kDa RNA
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Sep 22

Alu RNA pseudoknot alterations influence SRP9/SRP14 association. RNA (2025)
Gussakovsky D, Brown MJF, Pereira HS, Meier M, Padilla-Meier GP, Black NA, Booy EP, Stetefeld J, Patel TR, McKenna SA

R_g^Guinier	4.2	nm
D_max	13.6	nm
Volume^Porod	79	nm³

SASDWR3 – BC120 G25C RNA

Sample:	BC120 G25C RNA monomer, 39 kDa Homo sapiens RNA
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Sep 22

Alu RNA pseudoknot alterations influence SRP9/SRP14 association. RNA (2025)
Gussakovsky D, Brown MJF, Pereira HS, Meier M, Padilla-Meier GP, Black NA, Booy EP, Stetefeld J, Patel TR, McKenna SA

R_g^Guinier	4.1	nm
D_max	13.8	nm
Volume^Porod	63	nm³

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