Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDS79 – Long SARS-CoV-2 Non-structural protein 10 (Nsp10: C-terminal truncation variant)

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) experimental SAS data

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) Kratky plot

Sample:	Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) monomer, 14 kDa Severe acute respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Nov 25

Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F

R_g^Guinier	1.6	nm
D_max	5.4	nm
Volume^Porod	19	nm³

SASDS89 – Short SARS-CoV-2 Non-structural protein 10 (Nsp10: N- and C-terminal truncation variant)

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) experimental SAS data

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) Kratky plot

Sample:	Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) monomer, 13 kDa Severe acute respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Nov 25

R_g^Guinier	1.5	nm
D_max	5.1	nm
Volume^Porod	16	nm³

SASDS99 – Full length SARS Non-structural protein 10 (Nsp10)

Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data

Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot

Sample:	Replicase polyprotein 1ab (Non-structural protein 10) monomer, 16 kDa Severe acute respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 6

R_g^Guinier	1.7	nm
D_max	5.9	nm
Volume^Porod	19	nm³

SASDSA9 – Full length MERS Non-structural protein 10 (Nsp10)

Sample:	Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Middle East respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 6

R_g^Guinier	1.7	nm
D_max	5.6	nm
Volume^Porod	19	nm³

SASDSU6 – Palivizumab IgG

Sample:	Palivizumab IgG monomer, 145 kDa Commercial (Synagis) protein
Buffer:	PBS (137 mM NaCl, 2.7 mM KCl, 12 mM HPO4 2−/H2PO4 −), pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14

Respiratory syncytial virus (RSV)-approved monoclonal antibody Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors Journal of Biological Chemistry :105270 (2023)
Ettich J, Wittich C, Moll J, Behnke K, Floss D, Reiners J, Christmann A, Lang P, Smits S, Kolmar H, Scheller J

R_g^Guinier	5.0	nm
D_max	16.8	nm
Volume^Porod	252	nm³

SASDSV6 – Nanobody AIP1 VHH

Anti-Idiotypic-Palivizumab-Nanobody1 experimental SAS data

Sample:	Anti-Idiotypic-Palivizumab-Nanobody1 monomer, 20 kDa Lama glama protein
Buffer:	PBS (137 mM NaCl, 2.7 mM KCl, 12 mM HPO4 2−/H2PO4 −), pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14

R_g^Guinier	2.6	nm
D_max	8.8	nm
Volume^Porod	31	nm³

SASDSW6 – Palivizumab Nanobody AIP1VHH complex

Palivizumab IgGAnti-Idiotypic-Palivizumab-Nanobody1 experimental SAS data

Sample:	Palivizumab IgG monomer, 145 kDa Commercial (Synagis) protein Anti-Idiotypic-Palivizumab-Nanobody1 monomer, 20 kDa Lama glama protein
Buffer:	PBS (137 mM NaCl, 2.7 mM KCl, 12 mM HPO4 2−/H2PO4 −), pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14

R_g^Guinier	6.0	nm
D_max	20.4	nm

SASDKP7 – Probable transcriptional regulatory protein Lrp/AsnC protein Rv2324

Probable transcriptional regulatory protein (Probably AsnC-family) experimental SAS data

Sample:	Probable transcriptional regulatory protein (Probably AsnC-family) dimer, 36 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Mar 1

Mycobacterium tuberculosis Rv2324 is a multifunctional feast/famine regulatory protein involved in growth, DNA replication and damage control. Int J Biol Macromol 252:126459 (2023)
Dubey S, Maurya RK, Shree S, Kumar S, Jahan F, Krishnan MY, Ramachandran R

R_g^Guinier	2.7	nm
D_max	11.6	nm
Volume^Porod	156	nm³

SASDKQ7 – Probable transcriptional regulatory protein Lrp/AsnC protein Rv2324 in the presence of methionine

Sample:	Probable transcriptional regulatory protein (Probably AsnC-family) octamer, 143 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM EDTA, 2 mM methionine, pH: 7.5
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Dec 5

R_g^Guinier	4.0	nm
D_max	13.8	nm
Volume^Porod	246	nm³

SASDSX6 – Aquifex aeolicus domain of unknown function 507 at 1 mg/mL

DUF507 family protein experimental SAS data

Sample:	DUF507 family protein monomer, 22 kDa Aquifex aeolicus (strain … protein
Buffer:	20 mM Hepes pH 7.2, 400 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 7

Crystal structure of domain of unknown function 507 (DUF507) reveals a new protein fold. Sci Rep 13(1):13496 (2023)
McKay CE, Cheng J, Tanner JJ

R_g^Guinier	2.3	nm
D_max	9.0	nm
Volume^Porod	39	nm³

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