Browse by MACROMOLECULE type: protein

SASDPX6 – Calmodulin bound to the FF3 domain of pre-mRNA-processing factor 40 homolog A (Prp40A)

Calmodulin-1Pre-mRNA-processing factor 40 homolog A experimental SAS data
MODELLER model
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Pre-mRNA-processing factor 40 homolog A monomer, 9 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Sep 23
Binding by calmodulin is coupled to transient unfolding of the third FF domain of Prp40A. Protein Sci 32(4):e4606 (2023)
Díaz Casas A, Cordoba JJ, Ferrer BJ, Balakrishnan S, Wurm JE, Pastrana-Ríos B, Chazin WJ
RgGuinier 2.4 nm
Dmax 8.7 nm
VolumePorod 29 nm3

SASDQ87 – Solution structure of colibactin ClbK

Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK experimental SAS data
Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK Kratky plot
Sample: Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK dimer, 476 kDa Escherichia coli protein
Buffer: 50 mM Hepes pH 7.5, 200 mM NaCl, 5 mM DTT, 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Sep 14
Architecture of a PKS-NRPS hybrid megaenzyme involved in the biosynthesis of the genotoxin colibactin Structure (2023)
Bonhomme S, Contreras-Martel C, Dessen A, Macheboeuf P
RgGuinier 8.0 nm
Dmax 30.0 nm
VolumePorod 1340 nm3

SASDFY4 – R4-15 human dystrophin fragment

Human dystrophin central domain R4-15 fragment experimental SAS data
Human dystrophin central domain R4-15 fragment Kratky plot
Sample: Human dystrophin central domain R4-15 fragment monomer, 150 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2016 May 26
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 9.7 nm
Dmax 47.5 nm

SASDFZ4 – R16-24 human dystrophin fragment

Human dystrophin central domain R16-24 fragment experimental SAS data
Human dystrophin central domain R16-24 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 fragment monomer, 127 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Dec 17
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 9.3 nm
Dmax 34.0 nm

SASDF25 – R16-24 del45-55 human dystrophin fragment

Human dystrophin central domain R16-24 del45-55 fragment experimental SAS data
Human dystrophin central domain R16-24 del45-55 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 del45-55 fragment monomer, 58 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Sep 28
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 4.7 nm
Dmax 18.3 nm

SASDF35 – R16-24 del45-51 human dystrophin fragment

Human dystrophin central domain R16-24 del45-51 fragment experimental SAS data
Human dystrophin central domain R16-24 del45-51 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 del45-51 fragment monomer, 85 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Jul 13
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 7.0 nm
Dmax 26.2 nm

SASDGM2 – R16-24 del44-54 human dystrophin fragment

Human dystrophin central domain R16-24 del44-54 fragment experimental SAS data
Human dystrophin central domain R16-24 del44-54 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 del44-54 fragment monomer, 57 kDa Homo sapiens protein
Buffer: 20 mM Na-phosphate, 300 mM NaCl, 1 mM EDTA, 2% glycerol,, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 8
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 6.4 nm
Dmax 25.0 nm
VolumePorod 159 nm3

SASDRA6 – Cell division protein FtsQ N-terminal soluble domain

Cell division protein FtsQ experimental SAS data
Cell division protein FtsQ Kratky plot
Sample: Cell division protein FtsQ monomer, 11 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM phosphate buffer, 25 mM NaCl, pH: 6.5
Experiment: SAXS data collected at 5-ID-D DND-CAT, Advanced Photon Source (APS), Argonne National Laboratory on 2017 Jun 28
An Arg/Ala-rich helix in the N-terminal region of M. tuberculosis FtsQ is a potential membrane anchor of the Z-ring. Commun Biol 6(1):311 (2023)
Smrt ST, Escobar CA, Dey S, Cross TA, Zhou HX
RgGuinier 2.6 nm
Dmax 8.5 nm
VolumePorod 21 nm3

SASDQ59 – Mutant 7-deazapurine in DNA protein A (D95A)

DNA-guanine transglycosylase - D95A mutant experimental SAS data
GROMACS model
Sample: DNA-guanine transglycosylase - D95A mutant monomer, 50 kDa Salmonella enterica subsp. … protein
Buffer: 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22
7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system. Nucleic Acids Res (2023)
Gedara SH, Wood E, Gustafson A, Liang C, Hung SH, Savage J, Phan P, Luthra A, de Crécy-Lagard V, Dedon P, Swairjo MA, Iwata-Reuyl D
RgGuinier 2.7 nm
Dmax 10.1 nm
VolumePorod 67 nm3

SASDQ89 – 7-deazapurine in DNA protein B

DGQHR domain-containing protein experimental SAS data
ALPHAFOLD model
Sample: DGQHR domain-containing protein dimer, 88 kDa Salmonella enterica subsp. … protein
Buffer: 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22
7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system. Nucleic Acids Res (2023)
Gedara SH, Wood E, Gustafson A, Liang C, Hung SH, Savage J, Phan P, Luthra A, de Crécy-Lagard V, Dedon P, Swairjo MA, Iwata-Reuyl D
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 126 nm3