Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDJ24 – Immunoglobulin HUI-018 Fab and OXI-005 Fab in a ternary complex with porcine insulin

HUI-018 FabInsulin (Insulin B chain and Insulin A chain)OXI-005 Fab experimental SAS data

Sample:	HUI-018 Fab monomer, 47 kDa Mus musculus protein Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Sus scrofa protein OXI-005 Fab monomer, 47 kDa Mus musculus protein
Buffer:	20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at Rigaku BioSAXS-2000, Novo Nordisk A/S on 2019 Mar 28

Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
Johansson E, Wu X, Yu B, Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F

R_g^Guinier	4.5	nm
D_max	14.2	nm
Volume^Porod	138	nm³

SASDJ84 – Intimin D00-D0 domain

Intimin, D00-D0 domain (6xHis tagged) experimental SAS data

Sample:	Intimin, D00-D0 domain (6xHis tagged) monomer, 23 kDa Escherichia coli O127:H6 … protein
Buffer:	10 mM HEPES, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 12

The extracellular juncture domains in the intimin passenger adopt a constitutively extended conformation inducing restraints to its sphere of action. Sci Rep 10(1):21249 (2020)
Weikum J, Kulakova A, Tesei G, Yoshimoto S, Jægerum LV, Schütz M, Hori K, Skepö M, Harris P, Leo JC, Morth JP

R_g^Guinier	3.1	nm
D_max	8.3	nm
Volume^Porod	32	nm³

SASDJ94 – Intimin D0-D1 domain

Intimin (D0-D1 domain, 6xHis tagged) experimental SAS data

Sample:	Intimin (D0-D1 domain, 6xHis tagged) monomer, 23 kDa Escherichia coli O127:H6 … protein
Buffer:	10 mM HEPES, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 12

R_g^Guinier	2.2	nm
D_max	5.8	nm
Volume^Porod	34	nm³

SASDGU9 – Nucleolar RNA helicase 2 (DDX21-FL)

Nucleolar RNA helicase 2 experimental SAS data

Sample:	Nucleolar RNA helicase 2 dimer, 182 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2017 Nov 25

The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity iScience 23(12):101811 (2020)
Marcaida M, Kauzlaric A, Duperrex A, Sülzle J, Moncrieffe M, Adebajo D, Manley S, Trono D, Dal Peraro M

R_g^Guinier	7.0	nm
D_max	31.9	nm
Volume^Porod	681	nm³

SASDGV9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-783

Nucleolar RNA helicase 2 fragment 186-783 experimental SAS data

Sample:	Nucleolar RNA helicase 2 fragment 186-783 dimer, 138 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Mar 4

R_g^Guinier	4.8	nm
D_max	17.6	nm
Volume^Porod	297	nm³

SASDGW9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-710

Nucleolar RNA helicase 2 fragment 186-710 experimental SAS data

Nucleolar RNA helicase 2 (DDX21) fragment 186-710 Rg histogram

Sample:	Nucleolar RNA helicase 2 fragment 186-710 dimer, 121 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Mar 4

R_g^Guinier	4.6	nm
D_max	17.7	nm
Volume^Porod	238	nm³

SASDGX9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-620

Nucleolar RNA helicase 2 fragment 186-620 experimental SAS data

Sample:	Nucleolar RNA helicase 2 fragment 186-620 dimer, 98 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Mar 4

R_g^Guinier	4.4	nm
D_max	16.9	nm
Volume^Porod	150	nm³

SASDGY9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-783 monomeric mutant

Sample:	Nucleolar RNA helicase 2 monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Nov 3

R_g^Guinier	4.1	nm
D_max	17.7	nm
Volume^Porod	111	nm³

SASDH89 – Mitochondrial TIM8·13 chaperone in complex with the Tim23 membrane protein precursor

Mitochondrial import inner membrane translocase subunit TIM8Mitochondrial import inner membrane translocase subunit TIM13Mitochondrial import inner membrane translocase subunit TIM23 experimental SAS data

Sample:	Mitochondrial import inner membrane translocase subunit TIM8 trimer, 29 kDa Saccharomyces cerevisiae protein Mitochondrial import inner membrane translocase subunit TIM13 trimer, 34 kDa Saccharomyces cerevisiae protein Mitochondrial import inner membrane translocase subunit TIM23 monomer, 24 kDa Saccharomyces cerevisiae protein
Buffer:	50 mM Tris, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2015 Apr 24

Structural basis of client specificity in mitochondrial membrane-protein chaperones. Sci Adv 6(51) (2020)
Sučec I, Wang Y, Dakhlaoui O, Weinhäupl K, Jores T, Costa D, Hessel A, Brennich M, Rapaport D, Lindorff-Larsen K, Bersch B, Schanda P

R_g^Guinier	3.1	nm
D_max	11.3	nm

SASDJP4 – Mitochondrial import inner membrane translocase subunits TIM9-TIM10 in complex with TIM23

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10Mitochondrial import inner membrane translocase subunit TIM23 experimental SAS data

Sample:	Mitochondrial import inner membrane translocase subunit TIM9 trimer, 31 kDa Saccharomyces cerevisiae protein Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein Mitochondrial import inner membrane translocase subunit TIM23 monomer, 24 kDa Saccharomyces cerevisiae protein
Buffer:	50 mM Tris, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 18

R_g^Guinier	3.2	nm
D_max	7.7	nm
Volume^Porod	146	nm³

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