Browse by MACROMOLECULE type: protein

SASDGE5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95)

PDZ1-2 fragment of PSD-95/Disks large homolog 4 experimental SAS data
OTHER model
Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM TRIS/HCl, 150 mM NaCl, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 11
The dual PDZ domain from Postsynaptic density protein 95 forms a scaffold with peptide ligand Biophysical Journal (2020)
Rodzli N, Lockhart-Cairns M, Levy C, Chipperfield J, Bird L, Baldock C, Prince S
RgGuinier 2.4 nm
Dmax 9.2 nm
VolumePorod 32 nm3

SASDGF5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) (diluted)

PDZ1-2 fragment of PSD-95/Disks large homolog 4 experimental SAS data
OTHER model
Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM TRIS/HCl, 150 mM NaCl, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 11
The dual PDZ domain from Postsynaptic density protein 95 forms a scaffold with peptide ligand Biophysical Journal (2020)
Rodzli N, Lockhart-Cairns M, Levy C, Chipperfield J, Bird L, Baldock C, Prince S
RgGuinier 2.5 nm
Dmax 9.5 nm
VolumePorod 32 nm3

SASDGG5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) (Paused SEC)

PDZ1-2 fragment of PSD-95/Disks large homolog 4 experimental SAS data
OTHER model
Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM TRIS/HCl, 150 mM NaCl, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jan 26
The dual PDZ domain from Postsynaptic density protein 95 forms a scaffold with peptide ligand Biophysical Journal (2020)
Rodzli N, Lockhart-Cairns M, Levy C, Chipperfield J, Bird L, Baldock C, Prince S
RgGuinier 2.4 nm
Dmax 6.4 nm
VolumePorod 31 nm3

SASDGH5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) + glutathione (GSH)

PDZ1-2 fragment of PSD-95/Disks large homolog 4 experimental SAS data
OTHER model
Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM TRIS/HCl, 150 mM NaCl + 10 mM GSH, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Sep 16
The dual PDZ domain from Postsynaptic density protein 95 forms a scaffold with peptide ligand Biophysical Journal (2020)
Rodzli N, Lockhart-Cairns M, Levy C, Chipperfield J, Bird L, Baldock C, Prince S
RgGuinier 2.7 nm
Dmax 6.3 nm
VolumePorod 29 nm3

SASDGJ5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) + glutathione (GSH) (diluted)

PDZ1-2 fragment of PSD-95/Disks large homolog 4 experimental SAS data
OTHER model
Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM TRIS/HCl, 150 mM NaCl + 16 mM GSH, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Sep 16
The dual PDZ domain from Postsynaptic density protein 95 forms a scaffold with peptide ligand Biophysical Journal (2020)
Rodzli N, Lockhart-Cairns M, Levy C, Chipperfield J, Bird L, Baldock C, Prince S
RgGuinier 3.1 nm
Dmax 7.0 nm
VolumePorod 41 nm3

SASDGK5 – The PDZ1-2 domain of postsynaptic density protein 95 (PSD-95) + glutathione (GSH) (Paused SEC)

PDZ1-2 fragment of PSD-95/Disks large homolog 4 experimental SAS data
OTHER model
Sample: PDZ1-2 fragment of PSD-95/Disks large homolog 4 monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM TRIS/HCl, 150 mM NaCl + 10 mM GSH, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jan 26
The dual PDZ domain from Postsynaptic density protein 95 forms a scaffold with peptide ligand Biophysical Journal (2020)
Rodzli N, Lockhart-Cairns M, Levy C, Chipperfield J, Bird L, Baldock C, Prince S
RgGuinier 2.4 nm
Dmax 7.2 nm
VolumePorod 30 nm3

SASDFC4 – Condensin complex subunit 3-like protein, Ycg1, extrapolated to infinite dilution

Condensin complex subunit 3-like protein experimental SAS data
DAMMIF model
Sample: Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 4.6 nm
Dmax 15.6 nm
VolumePorod 236 nm3

SASDFD4 – Condensin complex subunit 3-like protein,Ycg1, bound to condensin complex subunit 2, Brn1 (Ycg1-Brn1)

Condensin complex subunit 3-like proteinCondensin complex subunit 2 experimental SAS data
DAMMIF model
Sample: Condensin complex subunit 3-like protein monomer, 108 kDa Chaetomium thermophilum protein
Condensin complex subunit 2 monomer, 17 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 4.3 nm
Dmax 13.7 nm
VolumePorod 230 nm3

SASDFE4 – Condensin complex subunit 3-like protein, Ycg1, at 10mg/ml (tetramer)

Condensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein tetramer, 433 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 8.3 nm
Dmax 32.7 nm
VolumePorod 1080 nm3

SASDFF4 – Condensin complex subunit 3-like protein, Ycg1, at 5mg/ml

Condensin complex subunit 3-like proteinCondensin complex subunit 3-like protein experimental SAS data
SASREF MX model
Sample: Condensin complex subunit 3-like protein tetramer, 433 kDa Chaetomium thermophilum protein
Condensin complex subunit 3-like protein dimer, 217 kDa Chaetomium thermophilum protein
Buffer: 25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 20
Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1. J Biol Chem 294(37):13822-13829 (2019)
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI
RgGuinier 6.8 nm
Dmax 29.8 nm
VolumePorod 720 nm3