Browse by MACROMOLECULE type: protein

SASDGK9 – Apolipoprotein E4 (methylated) bound to 1 mg/mL heparin (SEC-SAXS)

Apolipoprotein E4Heparin experimental SAS data
Apolipoprotein E4 Heparin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Heparin monomer, 15 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 6.4 nm
Dmax 21.7 nm

SASDGL9 – Apolipoprotein E4 (methylated) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4Suramin experimental SAS data
Apolipoprotein E4 Suramin Kratky plot
Sample: Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 5.3 nm
Dmax 18.5 nm

SASDGM9 – Mutant Apolipoprotein E4 (K143A K146A) - (SEC-SAXS)

Apolipoprotein E4 (K143A K146A) mutant experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 5.8 nm
Dmax 20.2 nm

SASDGN9 – Mutant Apolipoprotein E4 (K143A K146A) bound to 1 mg/mL heparin (SEC-SAXS)

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data
Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 5.7 nm
Dmax 19.8 nm

SASDGP9 – Mutant Apolipoprotein E4 (K143A K146A) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data
Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot
Sample: Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jul 21
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 5.4 nm
Dmax 18.3 nm

SASDGQ9 – Truncated Apolipoprotein E4 (1-191) - (SEC-SAXS)

Apolipoprotein E4 (1-191) experimental SAS data
Apolipoprotein E4 (1-191) Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 2.3 nm
Dmax 7.6 nm

SASDGR9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mg/mL heparin (SEC-SAXS)

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 4.2 nm
Dmax 16.8 nm

SASDGS9 – Truncated Apolipoprotein E4 (1-191) bound to 1 mM Suramin (SEC-SAXS)

Apolipoprotein E4 (1-191)Suramin experimental SAS data
Apolipoprotein E4 (1-191) Suramin Kratky plot
Sample: Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Suramin monomer, 1 kDa
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 2.4 nm
Dmax 8.3 nm

SASDGT9 – Truncated Apolipoprotein E4 (1-191) bound to 110 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data
Heparin Apolipoprotein E4 (1-191) Kratky plot
Sample: Heparin monomer, 15 kDa
Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 3
Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft
RgGuinier 2.7 nm
Dmax 8.3 nm

SASDEW7 – C-terminal truncated bank vole prion protein (amino acids 90-231)

Major prion protein experimental SAS data
C-terminal truncated bank vole prion protein (amino acids 90-231) Rg histogram
Sample: Major prion protein monomer, 16 kDa Myodes glareolus protein
Buffer: 25 mM Ammonium Acetate, 250 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 25
Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain Biophysical Journal (2020)
Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G
RgGuinier 2.3 nm
Dmax 9.4 nm
VolumePorod 29 nm3