Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDCS9 – Ammonium transporter Ks-Amt5 (Batch)

ammonium sensor/transducer experimental SAS data

Sample:	Ammonium sensor/transducer trimer, 227 kDa Candidatus Kuenenia stuttgartiensis protein
Buffer:	20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Mar 2

Signaling ammonium across membranes through an ammonium sensor histidine kinase. Nat Commun 9(1):164 (2018)
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA

R_g^Guinier	4.9	nm
D_max	17.0	nm
Volume^Porod	482	nm³

SASDCY4 – RNase E 603-850

Sample:	RNase E 603-850 monomer, 30 kDa Escherichia coli protein
Buffer:	50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v), pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2014 Dec 5

Analysis of the natively unstructured RNA/protein-recognition core in the Escherichia coli RNA degradosome and its interactions with regulatory RNA/Hfq complexes. Nucleic Acids Res 46(1):387-402 (2018)
Bruce HA, Du D, Matak-Vinkovic D, Bandyra KJ, Broadhurst RW, Martin E, Sobott F, Shkumatov AV, Luisi BF

R_g^Guinier	5.3	nm
D_max	27.5	nm
Volume^Porod	139	nm³

SASDCZ4 – RNase E 603-850/ATP-dependent RNA helicase (RhlB) binary complex

RNase E 603-850ATP-dependent RNA helicase RhlB experimental SAS data

Sample:	RNase E 603-850 monomer, 30 kDa Escherichia coli protein ATP-dependent RNA helicase RhlB monomer, 47 kDa Escherichia coli protein
Buffer:	50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v), pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Feb 11

R_g^Guinier	5.4	nm
D_max	29.5	nm
Volume^Porod	183	nm³

SASDC25 – RNase E 603-850/ATP-dependent RNA helicase (RhlB)/enolase ternary complex

RNase E 603-850ATP-dependent RNA helicase RhlBEnolase experimental SAS data

Sample:	RNase E 603-850 monomer, 30 kDa Escherichia coli protein ATP-dependent RNA helicase RhlB monomer, 47 kDa Escherichia coli protein Enolase dimer, 91 kDa Escherichia coli protein
Buffer:	50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v), pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2014 Jul 16

R_g^Guinier	6.4	nm
D_max	30.5	nm
Volume^Porod	280	nm³

SASDCK7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399)

Sample:	Cytohesin-3 monomer, 40 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 13

Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG

R_g^Guinier	2.8	nm
D_max	11.2	nm
Volume^Porod	61	nm³

SASDCL7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-390)

Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-390) Rg histogram

Sample:	Cytohesin-3 monomer, 40 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.001 mM inositol 1,3,4,5-tetrakisphosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Mar 21

R_g^Guinier	2.8	nm
D_max	9.3	nm
Volume^Porod	64	nm³

SASDCM7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L fusion protein

Grp1 63-399 E161A 6GS Arf6 Q67L fusion protein experimental SAS data

Sample:	Grp1 63-399 E161A 6GS Arf6 Q67L fusion protein monomer, 61 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	3.3	nm
D_max	11.9	nm
Volume^Porod	93	nm³

SASDCN7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L His6 fusion protein

Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein experimental SAS data

Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein Kratky plot

Sample:	Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein monomer, 62 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	3.4	nm
D_max	13.6	nm
Volume^Porod	101	nm³

SASDCP7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L SUMO fusion protein

Grp1 63-399 E161A 6GS Arf6 Q67L SUMO fusion protein experimental SAS data

Sample:	Grp1 63-399 E161A 6GS Arf6 Q67L SUMO fusion protein monomer, 72 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	3.8	nm
D_max	14.5	nm
Volume^Porod	116	nm³

SASDCQ7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A Arf6 Q67L fusion protein

Grp1 63-399 E161A Arf6 Q67L fusion protein experimental SAS data

Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A Arf6 Q67L fusion protein Rg histogram

Sample:	Grp1 63-399 E161A Arf6 Q67L fusion protein monomer, 60 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	3.1	nm
D_max	11.6	nm
Volume^Porod	86	nm³

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