Browse by MACROMOLECULE type: protein

SASDCM6 – Small GTPase Rab5 conjugated with ubiquitin at K116

Monoubiquitinated Rab5 at K165 experimental SAS data
Small GTPase Rab5 conjugated with ubiquitin at K116 Rg histogram
Sample: Monoubiquitinated Rab5 at K165 monomer, 32 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2016 Nov 21
Site-specific monoubiquitination downregulates Rab5 by disrupting effector binding and guanine nucleotide conversion. Elife 6 (2017)
Shin D, Na W, Lee JH, Kim G, Baek J, Park SH, Choi CY, Lee S
RgGuinier 2.6 nm
Dmax 8.7 nm

SASDCN6 – Small GTPase Rab5 conjugated with ubiquitin at K140

Monoubiquitinated Rab5 at K165 experimental SAS data
Small GTPase Rab5 conjugated with ubiquitin at K140 Rg histogram
Sample: Monoubiquitinated Rab5 at K165 monomer, 32 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2016 Nov 21
Site-specific monoubiquitination downregulates Rab5 by disrupting effector binding and guanine nucleotide conversion. Elife 6 (2017)
Shin D, Na W, Lee JH, Kim G, Baek J, Park SH, Choi CY, Lee S
RgGuinier 2.3 nm
Dmax 8.4 nm

SASDCP6 – Small GTPase Rab5 conjugated with ubiquitin at K165

Monoubiquitinated Rab5 at K165 experimental SAS data
Small GTPase Rab5 conjugated with ubiquitin at K165 Rg histogram
Sample: Monoubiquitinated Rab5 at K165 monomer, 32 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2016 Nov 21
Site-specific monoubiquitination downregulates Rab5 by disrupting effector binding and guanine nucleotide conversion. Elife 6 (2017)
Shin D, Na W, Lee JH, Kim G, Baek J, Park SH, Choi CY, Lee S
RgGuinier 2.7 nm
Dmax 9.4 nm

SASDBJ9 – Geobacillus stearothermophilus DnaB1-300

Geobacillus stearothermophilus DnaB1-300 experimental SAS data
GASBOR model
Sample: Geobacillus stearothermophilus DnaB1-300 tetramer, 138 kDa Geobacillus stearothermophilus protein
Buffer: 20 mM Tris, 300 mM NaCl and 5 mM β-ME, pH: 8
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2015 Oct 18
Structural analyses of the bacterial primosomal protein DnaB reveal that it is a tetramer and forms a complex with a primosomal re-initiation protein. J Biol Chem 292(38):15744-15757 (2017)
Li YC, Naveen V, Lin MG, Hsiao CD
RgGuinier 3.5 nm
Dmax 11.0 nm
VolumePorod 315 nm3

SASDBK9 – Geobacillus stearothermophilus DnaB full-length

Geobacillus stearothermophilus DnaB full-length experimental SAS data
GASBOR model
Sample: Geobacillus stearothermophilus DnaB full-length tetramer, 214 kDa Geobacillus stearothermophilus protein
Buffer: 20 mM Tris, 300 mM NaCl and 5 mM β-ME, pH: 8
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2015 Mar 12
Structural analyses of the bacterial primosomal protein DnaB reveal that it is a tetramer and forms a complex with a primosomal re-initiation protein. J Biol Chem 292(38):15744-15757 (2017)
Li YC, Naveen V, Lin MG, Hsiao CD
RgGuinier 5.7 nm
Dmax 20.0 nm
VolumePorod 432 nm3

SASDX22 – chloroplast trigger factor from Chlamydomonas reinhardtii

peptidylprolyl isomerase experimental SAS data
DAMMIN model
Sample: Peptidylprolyl isomerase monomer, 54 kDa Chlamydomonas reinhardtii protein
Buffer: 20 mM Tris pH 7.5, 150 mM KCl, pH:
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 30
Structural and molecular comparison of bacterial and eukaryotic trigger factors. Sci Rep 7(1):10680 (2017)
Ries F, Carius Y, Rohr M, Gries K, Keller S, Lancaster CRD, Willmund F
RgGuinier 3.9 nm
Dmax 12.5 nm
VolumePorod 103 nm3

SASDX32 – chloroplast trigger factor from Arabidopsis thaliana

Trigger factor-like protein TIG, Chloroplastic experimental SAS data
DAMMIN model
Sample: Trigger factor-like protein TIG, Chloroplastic monomer, 53 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris pH 7.5, 150 mM KCl, pH:
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 30
Structural and molecular comparison of bacterial and eukaryotic trigger factors. Sci Rep 7(1):10680 (2017)
Ries F, Carius Y, Rohr M, Gries K, Keller S, Lancaster CRD, Willmund F
RgGuinier 3.8 nm
Dmax 12.7 nm
VolumePorod 104 nm3

SASDBG2 – Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family).

Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). experimental SAS data
DAMMIN model
Sample: Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). dimer, 26 kDa Sulfolobus acidocaldarius protein
Buffer: 20 mM HEPES 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27
Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius. Mol Microbiol 105(5):777-793 (2017)
Li L, Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV
RgGuinier 2.6 nm
Dmax 9.8 nm
VolumePorod 47 nm3

SASDBH2 – Mutant archaeal biofilm regulator 1 (ABfR1) phosphomimic Y84E S87D

Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant experimental SAS data
DAMMIN model
Sample: Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant dimer, 26 kDa Sulfolobus acidocaldarius protein
Buffer: 20 mM HEPES 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27
Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius. Mol Microbiol 105(5):777-793 (2017)
Li L, Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV
RgGuinier 2.6 nm
Dmax 9.5 nm
VolumePorod 51 nm3

SASDCK2 – Glucose Isomerase - Streptomyces rubiginosus

Xylose isomerase experimental SAS data
DAMMIN model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, Tainer JA, Vachette P, Westbrook J, Whitten AE
RgGuinier 3.3 nm
Dmax 9.2 nm
VolumePorod 229 nm3