Browse by MACROMOLECULE type: protein

SASDBV4 – Vaccinia virus MVA F1L antiapoptotic Bcl-2 viral protein

MVA F1L antiapoptotic Bcl-2 viral protein experimental SAS data
BUNCH model
Sample: MVA F1L antiapoptotic Bcl-2 viral protein dimer, 51 kDa Vaccinia virus protein
Buffer: 25 mM HEPES 150 mM NaCl 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Apr 15
The N Terminus of the Vaccinia Virus Protein F1L Is an Intrinsically Unstructured Region That Is Not Involved in Apoptosis Regulation. J Biol Chem 291(28):14600-8 (2016)
Caria S, Marshall B, Burton RL, Campbell S, Pantaki-Eimany D, Hawkins CJ, Barry M, Kvansakul M
RgGuinier 3.4 nm
Dmax 16.2 nm
VolumePorod 74 nm3

SASDB54 – Leishmania braziliensis stress-induced protein sti1 (LbHop), full length construct

Stress-induced protein sti1 experimental SAS data
DAMFILT model
Sample: Stress-induced protein sti1 monomer, 62 kDa Leishmania braziliensis protein
Buffer: 25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2016 Feb 21
Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis. Arch Biochem Biophys 600:12-22 (2016)
Batista FAH, Seraphim TV, Santos CA, Gonzaga MR, Barbosa LRS, Ramos CHI, Borges JC
RgGuinier 4.5 nm
Dmax 18.0 nm
VolumePorod 94 nm3

SASDB64 – Leishmania braziliensis stress-induced protein sti1 (LbHop TPR2A-TPR2B-DP2 construct)

Stress-induced protein sti1 (Hop TPR2A-TPR2B-DP2 construct) experimental SAS data
DAMFILT model
Sample: Stress-induced protein sti1 (Hop TPR2A-TPR2B-DP2 construct) monomer, 44 kDa Leishmania braziliensis protein
Buffer: 25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2016 Feb 21
Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis. Arch Biochem Biophys 600:12-22 (2016)
Batista FAH, Seraphim TV, Santos CA, Gonzaga MR, Barbosa LRS, Ramos CHI, Borges JC
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 65 nm3

SASDB52 – Death associated protein kinase (wild-type)

Death associated protein kinase wild-type experimental SAS data
NONE model
Sample: Death associated protein kinase wild-type, 37 kDa Homo sapiens protein
Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
Simon B, Huart AS, Temmerman K, Vahokoski J, Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
RgGuinier 2.7 nm
Dmax 8.1 nm
VolumePorod 101 nm3

SASDB62 – Death associated protein kinase (D220K mutant)

Death associated protein kinase (D220K mutant) experimental SAS data
NONE model
Sample: Death associated protein kinase (D220K mutant), 37 kDa Homo sapiens protein
Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
Simon B, Huart AS, Temmerman K, Vahokoski J, Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 89 nm3

SASDB72 – Death associated protein kinase (Basic Loop mutant)

Death associated protein kinase (Basic Loop mutant) experimental SAS data
NONE model
Sample: Death associated protein kinase (Basic Loop mutant), 37 kDa Homo sapiens protein
Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
Simon B, Huart AS, Temmerman K, Vahokoski J, Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
RgGuinier 2.4 nm
Dmax 7.5 nm
VolumePorod 78 nm3

SASDAU8 – Immunosuppressive virulence protein YopM

Yersinia outer protein M (34-481) experimental SAS data
SASREF model
Sample: Yersinia outer protein M (34-481) dimer, 101 kDa Yersinia enterocolitica protein
Buffer: 50 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 13
Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells. PLoS Pathog 12(6):e1005660 (2016)
Berneking L, Schnapp M, Rumm A, Trasak C, Ruckdeschel K, Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M
RgGuinier 3.9 nm
Dmax 11.5 nm
VolumePorod 154 nm3

SASDAV8 – DEAD box helicase DDX3

DEAD box RNA helicase DDX3 (51-418) experimental SAS data
SASREF model
Sample: DEAD box RNA helicase DDX3 (51-418) monomer, 42 kDa Yersinia enterocolitica protein
Buffer: 50 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 13
Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells. PLoS Pathog 12(6):e1005660 (2016)
Berneking L, Schnapp M, Rumm A, Trasak C, Ruckdeschel K, Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M
RgGuinier 2.8 nm
Dmax 12.0 nm
VolumePorod 80 nm3

SASDAW8 – 2:1 YopM:DDX3 complex

Yersinia outer protein M (34-481)DEAD box RNA helicase DDX3 (51-418) experimental SAS data
SASREF model
Sample: Yersinia outer protein M (34-481) dimer, 101 kDa Yersinia enterocolitica protein
DEAD box RNA helicase DDX3 (51-418) monomer, 42 kDa Yersinia enterocolitica protein
Buffer: 50 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 14
Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells. PLoS Pathog 12(6):e1005660 (2016)
Berneking L, Schnapp M, Rumm A, Trasak C, Ruckdeschel K, Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M
RgGuinier 4.0 nm
Dmax 15.0 nm
VolumePorod 190 nm3

SASDBL3 – Highly similar to Actin cross-linking family protein 7 (ACF7) Homo Sapiens

cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 experimental SAS data
cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Kratky plot
Sample: CDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 monomer, 46 kDa Homo sapiens protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 10
In vivo epidermal migration requires focal adhesion targeting of ACF7. Nat Commun 7:11692 (2016)
Yue J, Zhang Y, Liang WG, Gou X, Lee P, Liu H, Lyu W, Tang WJ, Chen SY, Yang F, Liang H, Wu X
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 53 nm3