Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDBC4 – Plakin domain of Human plectin (spectrin repeats: SR3-SR9)

Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 experimental SAS data

Sample:	Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 monomer, 96 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 13

The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape. J Biol Chem 291(36):18643-62 (2016)
Ortega E, Manso JA, Buey RM, Carballido AM, Carabias A, Sonnenberg A, de Pereda JM

R_g^Guinier	8.5	nm
D_max	35.0	nm
Volume^Porod	135	nm³

SASDBD4 – Plakin domain fragment of Human desmoplakin (C-terminal region spectrin repeats: SR7-SR8-SR9)

Plakin domain fragment of Human Desmoplakin encompassing spectrin repeats SR7-SR8-SR9 experimental SAS data

Sample:	Plakin domain fragment of Human Desmoplakin encompassing spectrin repeats SR7-SR8-SR9 monomer, 42 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Sep 25

R_g^Guinier	4.4	nm
D_max	17.5	nm
Volume^Porod	69	nm³

SASDBE4 – Plakin domain of Human desmoplakin

Plakin domain of Human Desmoplakin experimental SAS data

Plakin domain of Human desmoplakin Rg histogram

Sample:	Plakin domain of Human Desmoplakin monomer, 99 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Sep 25

R_g^Guinier	7.5	nm
D_max	35.0	nm
Volume^Porod	136	nm³

SASDAC6 – PsrP functional binding region

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein experimental SAS data

PsrP functional binding region Rg histogram

Sample:	Functional binding region (187-385) of the pneumococcal serine-rich repeat protein monomer, 22 kDa Streptococcus pneumoniae protein
Buffer:	PBS 5 % Glycerol, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2013 Feb 27

The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation. Sci Rep 6:32371 (2016)
Schulte T, Mikaelsson C, Beaussart A, Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A

R_g^Guinier	2.0	nm
D_max	7.8	nm
Volume^Porod	38	nm³

SASDAE6 – PsrP functional binding region

Functional binding region (120-395) of the pneumococcal serine-rich repeat protein experimental SAS data

Sample:	Functional binding region (120-395) of the pneumococcal serine-rich repeat protein monomer, 30 kDa Streptococcus pneumoniae protein
Buffer:	PBS 5 % Glycerol, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2013 Feb 27

R_g^Guinier	2.9	nm
D_max	12.5	nm
Volume^Porod	41	nm³

SASDAP7 – PBX1-PREB1 complex

Pre-B-cell leukemia transcription factor 1Homeobox protein PKNOX1 experimental SAS data

Sample:	Pre-B-cell leukemia transcription factor 1 monomer, 34 kDa Homo sapiens protein Homeobox protein PKNOX1 monomer, 38 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6

The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding. FEBS J 283(16):3134-54 (2016)
Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C

R_g^Guinier	5.8	nm
D_max	19.0	nm
Volume^Porod	143	nm³

SASDBZ4 – Tyrosine hydroxylase (Isoform 1, Homo sapiens)

Tyrosine hydroxylase, isoform 1 experimental SAS data

Sample:	Tyrosine hydroxylase, isoform 1 tetramer, 222 kDa Homo sapiens protein
Buffer:	20 mM Na-HEPES 200 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Jun 14

Stable preparations of tyrosine hydroxylase provide the solution structure of the full-length enzyme. Sci Rep 6:30390 (2016)
Bezem MT, Baumann A, Skjærven L, Meyer R, Kursula P, Martinez A, Flydal MI

R_g^Guinier	4.7	nm
D_max	20.0	nm
Volume^Porod	520	nm³

SASDBY3 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state-TBE)

Aureochrome 1a bZIP-LOV module experimental SAS data

Sample:	Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer:	50 mM Tris 50 mM boric acid 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2014 Nov 6

Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. Nucleic Acids Res 44(12):5957-70 (2016)
Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T

R_g^Guinier	3.4	nm
D_max	12.6	nm
Volume^Porod	115	nm³

SASDB24 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state, Tris)

Aureochrome 1a bZIP-LOV module Kratky plot

Sample:	Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer:	10 mM Tris 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2014 Nov 6

R_g^Guinier	3.9	nm
D_max	12.5	nm
Volume^Porod	121	nm³

SASDB34 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (dark state, Tris)

Sample:	Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer:	10 mM Tris 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2014 Nov 6

R_g^Guinier	3.8	nm
D_max	12.5	nm
Volume^Porod	117	nm³

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