Browse by MACROMOLECULE type: protein

SASDB33 – Glutamate decarboxylase alpha (GadA) from E. coli

Glutamate decarboxylase alpha (GadA) from E. coli experimental SAS data
SASREF MX model
Sample: Glutamate decarboxylase alpha (GadA) from E. coli monomer, 53 kDa Escherichia coli protein
Buffer: 50 mM Tris 10 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 20
X-Ray Solution Scattering Study of Four Escherichia coli Enzymes Involved in Stationary-Phase Metabolism. PLoS One 11(5):e0156105 (2016)
Dadinova LA, Shtykova EV, Konarev PV, Rodina EV, Snalina NE, Vorobyeva NN, Kurilova SA, Nazarova TI, Jeffries CM, Svergun DI
RgGuinier 4.8 nm
VolumePorod 410 nm3

SASDBW3 – Human calumenin (sarco-endoplasmic reticulum calcium-sensing protein)

Human Calumenin experimental SAS data
Human Calumenin Kratky plot
Sample: Human Calumenin monomer, 29 kDa Homo sapiens protein
Buffer: 25 mM Na-HEPES, 25 mM NaCl, 2.5 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Feb 12
Ca-Dependent Folding of Human Calumenin. PLoS One 11(3):e0151547 (2016)
Mazzorana M, Hussain R, Sorensen T
RgGuinier 2.3 nm
Dmax 6.5 nm
VolumePorod 49 nm3

SASDBN4 – Callose synthase

Callose synthase experimental SAS data
CORAL model
Sample: Callose synthase octamer, 633 kDa Arabidopsis thaliana protein
Buffer: Tris, 50 mM NaCl, pH: 7.3
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Structural Characterization of Cell Wall and Plasma Membrane Proteins of Arabidopsis thaliana University of Hamburg Dissertation 8022 (2016)
Haifa El Kilani
RgGuinier 8.0 nm
Dmax 30.0 nm
VolumePorod 1033 nm3

SASDBS4 – Glutamate decarboxylase alpha (GadA) from E. coli, low salt

Glutamate decarboxylase alpha (GadA) from E. coli experimental SAS data
SASREF MX model
Sample: Glutamate decarboxylase alpha (GadA) from E. coli monomer, 53 kDa Escherichia coli protein
Buffer: 50 mM Tris, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jul 29
X-Ray Solution Scattering Study of Four Escherichia coli Enzymes Involved in Stationary-Phase Metabolism. PLoS One 11(5):e0156105 (2016)
Dadinova LA, Shtykova EV, Konarev PV, Rodina EV, Snalina NE, Vorobyeva NN, Kurilova SA, Nazarova TI, Jeffries CM, Svergun DI
RgGuinier 4.4 nm
VolumePorod 450 nm3

SASDBY5 – C-terminal fragment (509-716) of the Methoprene-tolerant protein from Drosophila melanogaster

FI10506p experimental SAS data
C-terminal fragment (509-716) of the Methoprene-tolerant protein from Drosophila melanogaster Rg histogram
Sample: FI10506p monomer, 23 kDa Drosophila melanogaster protein
Buffer: 20mM Tris/HCl 150mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jul 21
Intrinsic Disorder of the C-Terminal Domain of Drosophila Methoprene-Tolerant Protein. PLoS One 11(9):e0162950 (2016)
Kolonko M, Ożga K, Hołubowicz R, Taube M, Kozak M, Ożyhar A, Greb-Markiewicz B
RgGuinier 5.1 nm
Dmax 22.0 nm

SASDAM8 – MHV-68 LANA

Latency-associated nuclear antigen experimental SAS data
MHV-68 LANA Rg histogram
Sample: Latency-associated nuclear antigen tetramer, 87 kDa Murid herpesvirus 4 protein
Buffer: 25 mM Na/K Phosphate, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Apr 27
KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA. Nucleic Acids Res 43(20):10039-54 (2015)
Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE
RgGuinier 4.2 nm
Dmax 16.0 nm
VolumePorod 117 nm3

SASDAQ8 – kLANA mutant dimer-tetramer mixture

ORF73 tetramerORF73 dimer experimental SAS data
NONE model
Sample: ORF73 tetramer tetramer, 63 kDa Human herpesvirus 8 protein
ORF73 dimer dimer, 32 kDa Human herpesvirus 8 protein
Buffer: 25 mM Na/K Phosphate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Jun 21
KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA. Nucleic Acids Res 43(20):10039-54 (2015)
Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE
RgGuinier 2.4 nm
Dmax 9.5 nm
VolumePorod 50 nm3

SASDAC8 – SDS hydrolase SdsA1

Pseudomonas aeruginosa SDS hydrolase SdsA1 experimental SAS data
CRYSOL model
Sample: Pseudomonas aeruginosa SDS hydrolase SdsA1 dimer, 145 kDa Pseudomonas aeruginosa protein
Buffer: 50 mM HEPES, pH: 7
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2013 Jul 22
SdsA polymorph isolation and improvement of their crystal quality using nonconventional crystallization techniques Journal of Applied Crystallography 48(5):1551-1559 (2015)
De la Mora E, Flores-Hernández E, Jakoncic J, Stojanoff V, Siliqi D, Sánchez-Puig N, Moreno A
RgGuinier 3.6 nm
Dmax 16.9 nm
VolumePorod 261 nm3

SASDGM5 – Holo-RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA)

RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) experimental SAS data
OTHER model
Sample: RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM DTT, 4 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 May 31
Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion. Sci Rep 5:14223 (2015)
O'Brien DP, Hernandez B, Durand D, Hourdel V, Sotomayor-Pérez AC, Vachette P, Ghomi M, Chamot-Rooke J, Ladant D, Brier S, Chenal A
RgGuinier 4.4 nm
Dmax 15.5 nm
VolumePorod 89 nm3

SASDGN5 – apo-RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA)

RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) experimental SAS data
OTHER model
Sample: RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 May 31
Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion. Sci Rep 5:14223 (2015)
O'Brien DP, Hernandez B, Durand D, Hourdel V, Sotomayor-Pérez AC, Vachette P, Ghomi M, Chamot-Rooke J, Ladant D, Brier S, Chenal A
RgGuinier 8.3 nm
Dmax 33.0 nm